OBJECT: Bone morphogenetic proteins (BMPs) have been shown to have significant osteoinductive activity in numerous in vitro and in vivo assay systems, and BMP-2 and BMP-7 are currently being evaluated in human clinical studies. In the spinal region, BMPs have been shown to promote spinal arthrodesis at a higher rate than autologous bone alone. The delivery of BMPs via direct or ex vivo gene therapy techniques is also currently being evaluated and has shown promise in several mammalian models. The present study was designed to evaluate the efficacy of the use of direct, percutaneous BMP-9 adenoviral gene therapy to promote spinal fusion in the rodent. METHODS: Each animal was injected with 7.5x10(8) pfu of a BMP-9 adenoviral vector in the lumbar paraspinal musculature and allowed to survive 16 weeks. Computerized tomography studies and histological analysis demonstrated massive bone induction at the injection sites, clearly leading to solid spinal arthrodesis, without evidence of pseudarthroses, nerve root compression, or systemic side effects. CONCLUSIONS: The results of this study strongly support the advancement of BMP gene therapy techniques toward clinical use.
OBJECT: Bone morphogenetic proteins (BMPs) have been shown to have significant osteoinductive activity in numerous in vitro and in vivo assay systems, and BMP-2 and BMP-7 are currently being evaluated in human clinical studies. In the spinal region, BMPs have been shown to promote spinal arthrodesis at a higher rate than autologous bone alone. The delivery of BMPs via direct or ex vivo gene therapy techniques is also currently being evaluated and has shown promise in several mammalian models. The present study was designed to evaluate the efficacy of the use of direct, percutaneous BMP-9 adenoviral gene therapy to promote spinal fusion in the rodent. METHODS: Each animal was injected with 7.5x10(8) pfu of a BMP-9 adenoviral vector in the lumbar paraspinal musculature and allowed to survive 16 weeks. Computerized tomography studies and histological analysis demonstrated massive bone induction at the injection sites, clearly leading to solid spinal arthrodesis, without evidence of pseudarthroses, nerve root compression, or systemic side effects. CONCLUSIONS: The results of this study strongly support the advancement of BMP gene therapy techniques toward clinical use.
Authors: Ashvin K Dewan; Rahul A Dewan; Nathan Calderon; Angie Fuentes; Zawaunyka Lazard; Alan R Davis; Michael Heggeness; John A Hipp; Elizabeth A Olmsted-Davis Journal: J Orthop Surg Res Date: 2010-08-21 Impact factor: 2.359
Authors: Joseph D Lamplot; Jiaqiang Qin; Guoxin Nan; Jinhua Wang; Xing Liu; Liangjun Yin; Justin Tomal; Ruidong Li; Wei Shui; Hongyu Zhang; Stephanie H Kim; Wenwen Zhang; Jiye Zhang; Yuhan Kong; Sahitya Denduluri; Mary Rose Rogers; Abdullah Pratt; Rex C Haydon; Hue H Luu; Jovito Angeles; Lewis L Shi; Tong-Chuan He Journal: Am J Stem Cells Date: 2013-03-08
Authors: Maureen Beederman; Joseph D Lamplot; Guoxin Nan; Jinhua Wang; Xing Liu; Liangjun Yin; Ruidong Li; Wei Shui; Hongyu Zhang; Stephanie H Kim; Wenwen Zhang; Jiye Zhang; Yuhan Kong; Sahitya Denduluri; Mary Rose Rogers; Abdullah Pratt; Rex C Haydon; Hue H Luu; Jovito Angeles; Lewis L Shi; Tong-Chuan He Journal: J Biomed Sci Eng Date: 2013-08