Literature DB >> 10742260

Engineering desiccation tolerance in Escherichia coli.

D Billi1, D J Wright, R F Helm, T Prickett, M Potts, J H Crowe.   

Abstract

Recombinant sucrose-6-phosphate synthase (SpsA) was synthesized in Escherichia coli BL21DE3 by using the spsA gene of the cyanobacterium Synechocystis sp. strain PCC 6803. Transformants exhibited a 10,000-fold increase in survival compared to wild-type cells following either freeze-drying, air drying, or desiccation over phosphorus pentoxide. The phase transition temperatures and vibration frequencies (P==O stretch) in phospholipids suggested that sucrose maintained membrane fluidity during cell dehydration.

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Year:  2000        PMID： 10742260      PMCID： PMC92041          DOI： 10.1128/AEM.66.4.1680-1684.2000

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  17 in total

1.  Coupled enzymatic assay for the determination of sucrose.

Authors:  E W Holmes
Journal:  Anal Biochem       Date:  1997-01-01       Impact factor: 3.365

2.  Age of bacteria from amber.

Authors:  F G Priest
Journal:  Science       Date:  1995-12-22       Impact factor: 47.728

Review 3.  Preservation records of micro-organisms: evidence of the tenacity of life.

Authors:  M J Kennedy; S L Reader; L M Swierczynski
Journal:  Microbiology (Reading)       Date:  1994-10       Impact factor: 2.777

4.  Staphylococcus succinus sp. nov., isolated from Dominican amber.

Authors:  L H Lambert; T Cox; K Mitchell; R A Rosselló-Mora; C Del Cueto; D E Dodge; P Orkand; R J Cano
Journal:  Int J Syst Bacteriol       Date:  1998-04

5.  Effect of sugars on headgroup mobility in freeze-dried dipalmitoylphosphatidylcholine bilayers: solid-state 31P NMR and FTIR studies.

Authors:  N M Tsvetkova; B L Phillips; L M Crowe; J H Crowe; S H Risbud
Journal:  Biophys J       Date:  1998-12       Impact factor: 4.033

6.  Lipid phase transitions measured in intact cells with Fourier transform infrared spectroscopy.

Authors:  J H Crowe; F A Hoekstra; L M Crowe; T J Anchordoguy; E Drobnis
Journal:  Cryobiology       Date:  1989-02       Impact factor: 2.487

Review 7.  Desiccation tolerance of prokaryotes.

Authors:  M Potts
Journal:  Microbiol Rev       Date:  1994-12

8.  Is vitrification involved in depression of the phase transition temperature in dry phospholipids?

Authors:  J H Crowe; F A Hoekstra; K H Nguyen; L M Crowe
Journal:  Biochim Biophys Acta       Date:  1996-04-26

9.  Preservation of membranes in anhydrobiotic organisms: the role of trehalose.

Authors:  J H Crowe; L M Crowe; D Chapman
Journal:  Science       Date:  1984-02-17       Impact factor: 47.728

10.  Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying.

Authors:  S B Leslie; E Israeli; B Lighthart; J H Crowe; L M Crowe
Journal:  Appl Environ Microbiol       Date:  1995-10       Impact factor: 4.792
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  20 in total

1.  Anhydrobiotic engineering of gram-negative bacteria.

Authors:  A García De Castro; H Bredholt; A R Strøm; A Tunnacliffe
Journal:  Appl Environ Microbiol       Date:  2000-09       Impact factor: 4.792

2.  Unusual water flux in the extracellular polysaccharide of the cyanobacterium Nostoc commune.

Authors:  Eric Shaw; Donna R Hill; Nicole Brittain; Debbie J Wright; Uwe Täuber; Hervé Marand; Richard F Helm; Malcolm Potts
Journal:  Appl Environ Microbiol       Date:  2003-09       Impact factor: 4.792

Review 3.  Anhydrobiosis in bacteria: from physiology to applications.

Authors:  Armando Hernández García
Journal:  J Biosci       Date:  2011-12       Impact factor: 1.826

4.  Multiple-stress tolerance of ionizing radiation-resistant bacterial isolates obtained from various habitats: correlation between stresses.

Authors:  Manish Shukla; Ruchi Chaturvedi; Dhruti Tamhane; Pranav Vyas; G Archana; Shree Apte; J Bandekar; Anjana Desai
Journal:  Curr Microbiol       Date:  2006-12-18       Impact factor: 2.188

5.  Design-driven, multi-use research agendas to enable applied synthetic biology for global health.

Authors:  James M Carothers
Journal:  Syst Synth Biol       Date:  2013-07-20

6.  Bacterial growth at the high concentrations of magnesium sulfate found in martian soils.

Authors:  J D Crisler; T M Newville; F Chen; B C Clark; M A Schneegurt
Journal:  Astrobiology       Date:  2012-01-16       Impact factor: 4.335

Review 7.  Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering.

Authors:  Naira Quintana; Frank Van der Kooy; Miranda D Van de Rhee; Gerben P Voshol; Robert Verpoorte
Journal:  Appl Microbiol Biotechnol       Date:  2011-06-21       Impact factor: 4.813

8.  Ability of Shiga toxin-producing Escherichia coli and Salmonella spp. to survive in a desiccation model system and in dry foods.

Authors:  Reiji Hiramatsu; Masakado Matsumoto; Kenji Sakae; Yutaka Miyazaki
Journal:  Appl Environ Microbiol       Date:  2005-11       Impact factor: 4.792

9.  Proline biosynthesizing enzymes (glutamate 5-kinase and pyrroline-5-carboxylate reductase) from a model cyanobacterium for desiccation tolerance.

Authors:  Priyanka Singh; Anupam Tiwari; Sureshwar Prasad Singh; Ravi Kumar Asthana
Journal:  Physiol Mol Biol Plants       Date:  2013-10

10.  Hydroxyectoine is superior to trehalose for anhydrobiotic engineering of Pseudomonas putida KT2440.

Authors:  M Manzanera; A García de Castro; A Tøndervik; M Rayner-Brandes; A R Strøm; A Tunnacliffe
Journal:  Appl Environ Microbiol       Date:  2002-09       Impact factor: 4.792
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