Literature DB >> 10734206

Advanced method for oligonucleotide deprotection.

S A Surzhikov1, E N Timofeev, B K Chernov, J B Golova, A D Mirzabekov.   

Abstract

A new procedure for rapid deprotection of synthetic oligodeoxynucleotides has been developed. While all known deprotection methods require purification to remove the residual protective groups (e.g. benzamide) and insoluble silicates, the new procedure based on the use of an ammonia-free reagent mixture allows one to avoid the additional purification steps. The method can be applied to deprotect the oligodeoxynucleotides synthesized by using the standard protected nucleoside phosphoramidites dG(iBu), dC(Bz)and dA(Bz).

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Year:  2000        PMID： 10734206      PMCID： PMC102833          DOI： 10.1093/nar/28.8.e29

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  11 in total

1.  Regioselective immobilization of short oligonucleotides to acrylic copolymer gels.

Authors:  E Timofeev; S V Kochetkova; A D Mirzabekov; V L Florentiev
Journal:  Nucleic Acids Res       Date:  1996-08-15       Impact factor: 16.971

2.  Massive parallel analysis of DNA-Hoechst 33258 binding specificity with a generic oligodeoxyribonucleotide microchip.

Authors:  A L Drobyshev; A S Zasedatelev; G M Yershov; A D Mirzabekov
Journal:  Nucleic Acids Res       Date:  1999-10-15       Impact factor: 16.971

3.  DNA sequencing by hybridization to microchip octa-and decanucleotides extended by stacked pentanucleotides.

Authors:  S Parinov; V Barsky; G Yershov; E Kirillov; E Timofeev; A Belgovskiy; A Mirzabekov
Journal:  Nucleic Acids Res       Date:  1996-08-01       Impact factor: 16.971

4.  Sequence analysis by hybridization with oligonucleotide microchip: identification of beta-thalassemia mutations.

Authors:  A Drobyshev; N Mologina; V Shik; D Pobedimskaya; G Yershov; A Mirzabekov
Journal:  Gene       Date:  1997-03-25       Impact factor: 3.688

5.  Manual manufacturing of oligonucleotide, DNA, and protein microchips.

Authors:  D Guschin; G Yershov; A Zaslavsky; A Gemmell; V Shick; D Proudnikov; P Arenkov; A Mirzabekov
Journal:  Anal Biochem       Date:  1997-08-01       Impact factor: 3.365

6.  Labile exocyclic amine protection of nucleosides in DNA, RNA and oligonucleotide analog synthesis facilitating N-deacylation, minimizing depurination and chain degradation.

Authors:  N D Sinha; P Davis; N Usman; J Pérez; R Hodge; J Kremsky; R Casale
Journal:  Biochimie       Date:  1993       Impact factor: 4.079

7.  DNA analysis and diagnostics on oligonucleotide microchips.

Authors:  G Yershov; V Barsky; A Belgovskiy; E Kirillov; E Kreindlin; I Ivanov; S Parinov; D Guschin; A Drobishev; S Dubiley; A Mirzabekov
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-14       Impact factor: 11.205

8.  On the rapid deprotection of synthetic oligonucleotides and analogs.

Authors:  N N Polushin; A M Morocho; B C Chen; J S Cohen
Journal:  Nucleic Acids Res       Date:  1994-02-25       Impact factor: 16.971

9.  The final deprotection step in oligonucleotide synthesis is reduced to a mild and rapid ammonia treatment by using labile base-protecting groups.

Authors:  J C Schulhof; D Molko; R Teoule
Journal:  Nucleic Acids Res       Date:  1987-01-26       Impact factor: 16.971

10.  The isopropoxyacetic group for convenient base protection during solid-support synthesis of oligodeoxyribonucleotides and their triester analogs.

Authors:  B Uznanski; A Grajkowski; A Wilk
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971
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  1 in total

1.  Solid phase synthesis of a metronidazole oligonucleotide conjugate.

Authors:  Andrew J Walsh; Michael L Davis; William Fraser
Journal:  Molecules       Date:  2006-06-23       Impact factor: 4.411
  1 in total

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