Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Automation of NMR measurements and data evaluation for systematically screening interactions of small molecules with target proteins.

Literature DB >> 10723993

Automation of NMR measurements and data evaluation for systematically screening interactions of small molecules with target proteins.

A Ross¹, G Schlotterbeck, W Klaus, H Senn.

Abstract

In this technical note we describe the setup and application of automated sample preparation and usage of flow-through NMR equipment for the characterization of ligand binding on proteins. In addition, we focus on the perspectives of automated analysis of 2D HSQC spectra to identify changes in patterns indicative for ligand binding or changes of sample conditions. In this context we discuss a combination of statistical and non-statistical data analysis.

Mesh：

Substances：

Year: 2000 PMID： 10723993 DOI： 10.1023/a:1008394910612

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

20 in total

1. Solution structure of the SH3 domain of Src and identification of its ligand-binding site.

Authors: H Yu; M K Rosen; T B Shin; C Seidel-Dugan; J S Brugge; S L Schreiber
Journal: Science Date: 1992-12-04 Impact factor: 47.728

2. Fast-HMQC using Ernst angle pulses: An efficient tool for screening of ligand binding to target proteins.

Authors: A Ross; M Salzmann; H Senn
Journal: J Biomol NMR Date: 1997-12 Impact factor: 2.835

3. Protein hydration in aqueous solution.

Authors: G Otting; E Liepinsh; K Wüthrich
Journal: Science Date: 1991-11-15 Impact factor: 47.728

Review 4. Locating and characterizing binding sites on proteins.

Authors: C Mattos; D Ringe
Journal: Nat Biotechnol Date: 1996-05 Impact factor: 54.908

5. Screening mixtures for biological activity by NMR.

Authors: B Meyer; T Weimar; T Peters
Journal: Eur J Biochem Date: 1997-06-15

6. Mapping of the binding interfaces of the proteins of the bacterial phosphotransferase system, HPr and IIAglc.

Authors: Y Chen; J Reizer; M H Saier; W J Fairbrother; P E Wright
Journal: Biochemistry Date: 1993-01-12 Impact factor: 3.162

7. Organic solvents identify specific ligand binding sites on protein surfaces.

Authors: E Liepinsh; G Otting
Journal: Nat Biotechnol Date: 1997-03 Impact factor: 54.908

8. Linear prediction enhancement of 2D heteronuclear correlated spectra of proteins.

Authors: J J Led; H Gesmar
Journal: J Biomol NMR Date: 1991-09 Impact factor: 2.835

9. Solution studies of staphylococcal nuclease H124L. 2. 1H, 13C, and 15N chemical shift assignments for the unligated enzyme and analysis of chemical shift changes that accompany formation of the nuclease-thymidine 3',5'-bisphosphate-calcium ternary complex.

Authors: J F Wang; A P Hinck; S N Loh; D M LeMaster; J L Markley
Journal: Biochemistry Date: 1992-01-28 Impact factor: 3.162

10. 1H and 15N resonance assignments and secondary structure of cellular retinoic acid-binding protein with and without bound ligand.

Authors: J Rizo; Z P Liu; L M Gierasch
Journal: J Biomol NMR Date: 1994-11 Impact factor: 2.835

8 in total

1. Automated evaluation of chemical shift perturbation spectra: New approaches to quantitative analysis of receptor-ligand interaction NMR spectra.

Authors: Chen Peng; Stephen W Unger; Fabian V Filipp; Michael Sattler; Sándor Szalma
Journal: J Biomol NMR Date: 2004-08 Impact factor: 2.835

2. A general algorithm for peak-tracking in multi-dimensional NMR experiments.

Authors: P Ravel; G Kister; T E Malliavin; M A Delsuc
Journal: J Biomol NMR Date: 2007-02-10 Impact factor: 2.835

3. Modulation of the structure, catalytic activity, and fidelity of African swine fever virus DNA polymerase X by a reversible disulfide switch.

Authors: Markus W Voehler; Robert L Eoff; W Hayes McDonald; F Peter Guengerich; Michael P Stone
Journal: J Biol Chem Date: 2009-05-05 Impact factor: 5.157