OBJECTIVE: Cardiomyopathy, a popular diagnosis that always obscures more than it reveals, nevertheless has several characteristic histological features. These prominently include widespread focal myocardial fibrosis and associated hypertrophy of surviving cardiac myocyte. In fact, focal noninflammatory degeneration (not necrosis) has been demonstrated as a feature of many forms of cardiac hypertrophy. We hypothesized that this loss of myocardial cells in dilated cardiomyopathy (DCMP) may result from cell death by apoptosis. METHODS: Endomyocardial biopsy specimens from the right ventricles of six patients who suffered from DCMP were studied, and myocardial specimens from two persons who died in motor vehicle accidents were used as negative controls. For identification of apoptosis, immunohistochemistry with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end-labeling was performed. In addition, apoptosis was confirmed morphologically by confocal laser scanning microscopy with propidium iodide. RESULTS: Apoptosis, that was represented by an apoptotic index ranging from 19.8 to 25.4%, could be extensively seen in myocytes and also rarely in non-myocytes of interstitium and vascular endothelium. Morphologically, there were a lot of nuclei with clumps of condensed chromatin, suggestive of apoptosis. CONCLUSION: The present study demonstrated that myocyte loss in DCMP might be mainly due to the apoptosis of myocytes and interstitial cells, rather than inflammation or cell necrosis.
OBJECTIVE:Cardiomyopathy, a popular diagnosis that always obscures more than it reveals, nevertheless has several characteristic histological features. These prominently include widespread focal myocardial fibrosis and associated hypertrophy of surviving cardiac myocyte. In fact, focal noninflammatory degeneration (not necrosis) has been demonstrated as a feature of many forms of cardiac hypertrophy. We hypothesized that this loss of myocardial cells in dilated cardiomyopathy (DCMP) may result from cell death by apoptosis. METHODS: Endomyocardial biopsy specimens from the right ventricles of six patients who suffered from DCMP were studied, and myocardial specimens from two persons who died in motor vehicle accidents were used as negative controls. For identification of apoptosis, immunohistochemistry with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end-labeling was performed. In addition, apoptosis was confirmed morphologically by confocal laser scanning microscopy with propidium iodide. RESULTS: Apoptosis, that was represented by an apoptotic index ranging from 19.8 to 25.4%, could be extensively seen in myocytes and also rarely in non-myocytes of interstitium and vascular endothelium. Morphologically, there were a lot of nuclei with clumps of condensed chromatin, suggestive of apoptosis. CONCLUSION: The present study demonstrated that myocyte loss in DCMP might be mainly due to the apoptosis of myocytes and interstitial cells, rather than inflammation or cell necrosis.
Authors: J Kajstura; M Mansukhani; W Cheng; K Reiss; S Krajewski; J C Reed; F Quaini; E H Sonnenblick; P Anversa Journal: Exp Cell Res Date: 1995-07 Impact factor: 3.905
Authors: Weihong He; Charlotte S McCarroll; Katrin Nather; Kristopher Ford; Kenneth Mangion; Alexandra Riddell; Dylan O'Toole; Ali Zaeri; David Corcoran; David Carrick; Mathew M Y Lee; Margaret McEntegart; Andrew Davie; Richard Good; Mitchell M Lindsay; Hany Eteiba; Paul Rocchiccioli; Stuart Watkins; Stuart Hood; Aadil Shaukat; Lisa McArthur; Elspeth B Elliott; John McClure; Catherine Hawksby; Tamara Martin; Mark C Petrie; Keith G Oldroyd; Godfrey L Smith; Keith M Channon; Colin Berry; Stuart A Nicklin; Christopher M Loughrey Journal: Cardiovasc Res Date: 2022-05-06 Impact factor: 13.081
Authors: Ana Rodriguez; Karan Chawla; Nsini A Umoh; Valerie M Cousins; Assama Ketegou; Madhumati G Reddy; Mustafa AlRubaiee; Georges E Haddad; Mark W Burke Journal: Biomolecules Date: 2015-11-19