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Literature DB >> 1069992

Histones H3 and H4 interact with the ends of nucleosome DNA.

Abstract

Isolated HeLa cell nucleosomes (core particles) were labeled at the 5'-termini of their DNA with 32P using [gamma-32P]ATP and polynucleotide kne by sequential methylation, depurination, Schiff base formation, and reduction with sodium borhydride. After digestion of the noncrosslinked DNA by DNase I and venom phosphodiesterase, histones were separated by gel electrophoresis and those crosslinked to the 5'-termini were identified by 32P-autoradiography. Histones H3 and H4 occur with equeal frequency as the nearest protein neighbors to the end of the DNA in nucleosomes. Histone arrangements within the core particle compatible with these results are discussed.

Entities: Chemical

Mesh：

Substances：
Chromatin
Histones
DNA

Year: 1976 PMID： 1069992 PMCID： PMC431473 DOI： 10.1073/pnas.73.12.4400

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

35 in total

7. Protein kinase in HeLA nucleosomes: a reevaluation of the interactions of histomes with the ends of core particle DNA.

Authors: R T Simpson
Journal: Nucleic Acids Res Date: 1978-04 Impact factor: 16.971

8. K12-biotinylated histone H4 is enriched in telomeric repeats from human lung IMR-90 fibroblasts.

Authors: Subhashinee S K Wijeratne; Gabriela Camporeale; Janos Zempleni
Journal: J Nutr Biochem Date: 2009-04-14 Impact factor: 6.048

8 in total

Histones H3 and H4 interact with the ends of nucleosome DNA.

1. A model for chromatin sub-structure incorporating symmetry considerations of histone oligomers.

2. Cleavage of DNA in nuclei and chromatin with staphylococcal nuclease.

3. A comparison of the digestion of nuclei and chromatin by staphylococcal nuclease.

4. [Covalent binding of proteins with DNA in the chromatin structure].

5. Histone-histone associations within chromatin. Cross-linking studies using tetranitromethane.

6. Analysis of subunit organization in chicken erythrocyte chromatin.

7. Physical studies on the H3/H4 histone tetramer.

8. Removal of histone H1 exposes a fifty base pair DNA segment between nucleosomes.

9. An octamer of histones in chromatin and free in solution.

10. Histone compostion of chromatin subunits studied by immunosedimentation.

1. Conformational states of chromatin nu bodies induced by urea.

2. Chromatin core particle unfolding induced by tryptic cleavage of histones.

3. Histone packing in the nucleosome core particle of chromatin.

4. Photochemical cross-linking of histones to DNA nucleosomes.

5. Proximity and accessibility studies of histones in nuclei and free nucleosomes.

6. The helical model of the nucleosome core.

7. Protein kinase in HeLA nucleosomes: a reevaluation of the interactions of histomes with the ends of core particle DNA.

8. K12-biotinylated histone H4 is enriched in telomeric repeats from human lung IMR-90 fibroblasts.