Literature DB >> 10698991

Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance.

M Mehrotra1, G Wang, W M Johnson.   

Abstract

A multiplex PCR assay for detection of genes for staphylococcal enterotoxins A to E (entA, entB, entC, entD, and entE), toxic shock syndrome toxin 1 (tst), exfoliative toxins A and B (etaA and etaB), and intrinsic methicillin resistance (mecA) was developed. Detection of femA was used as an internal positive control. The multiplex PCR assay combined the primers for sea to see and femA in one set and those for eta, etb, tst, mecA, and femA in the other set. Validation of the assay was performed using 176 human isolates of Staphylococcus aureus. This assay offers a very specific, quick, reliable, and inexpensive alternative to conventional PCR assays used in clinical laboratories to identify various staphylococcal toxin genes.

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Year:  2000        PMID： 10698991      PMCID： PMC86330     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  30 in total

1.  Sequence comparison of mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis.

Authors:  C Ryffel; W Tesch; I Birch-Machin; P E Reynolds; L Barberis-Maino; F H Kayser; B Berger-Bächi
Journal:  Gene       Date:  1990-09-28       Impact factor: 3.688

2.  Detection of genes for enterotoxins, exfoliative toxins, and toxic shock syndrome toxin 1 in Staphylococcus aureus by the polymerase chain reaction.

Authors:  W M Johnson; S D Tyler; E P Ewan; F E Ashton; D R Pollard; K R Rozee
Journal:  J Clin Microbiol       Date:  1991-03       Impact factor: 5.948

3.  Rapid assay for detection of methicillin-resistant Staphylococcus aureus using multiplex PCR.

Authors:  P Barski; L Piechowicz; J Galiński; J Kur
Journal:  Mol Cell Probes       Date:  1996-12       Impact factor: 2.365

4.  Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D.

Authors:  K W Bayles; J J Iandolo
Journal:  J Bacteriol       Date:  1989-09       Impact factor: 3.490

5.  Nucleotide sequence of the type A staphylococcal enterotoxin gene.

Authors:  M J Betley; J J Mekalanos
Journal:  J Bacteriol       Date:  1988-01       Impact factor: 3.490

6.  Nucleotide sequence of the staphylococcal enterotoxin C1 gene and relatedness to other pyrogenic toxins.

Authors:  G A Bohach; P M Schlievert
Journal:  Mol Gen Genet       Date:  1987-08

Review 7.  Genetic analysis of extracellular toxins of Staphylococcus aureus.

Authors:  J J Iandolo
Journal:  Annu Rev Microbiol       Date:  1989       Impact factor: 15.500

Review 8.  Role of superantigens in human disease.

Authors:  P M Schlievert
Journal:  J Infect Dis       Date:  1993-05       Impact factor: 5.226

Review 9.  The staphylococcal enterotoxins and their relatives.

Authors:  P Marrack; J Kappler
Journal:  Science       Date:  1990-05-11       Impact factor: 47.728

10.  FemA, a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: molecular cloning and characterization.

Authors:  B Berger-Bächi; L Barberis-Maino; A Strässle; F H Kayser
Journal:  Mol Gen Genet       Date:  1989-10
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  156 in total

1.  Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus.

Authors:  Duarte C Oliveira; Hermínia de Lencastre
Journal:  Antimicrob Agents Chemother       Date:  2002-07       Impact factor: 5.191

2.  Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates.

Authors:  Astrid Løvseth; Semir Loncarevic; Knut G Berdal
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

3.  Prevalence of Enterotoxin Genes and spa Genotypes of Methicillin-resistant Staphylococcus aureus from a Tertiary Care Hospital in China.

Authors:  Yanmeng Li; Ruike Zhao; Xianfeng Zhang; Qingzhen Han; Xuefeng Qian; Guohao Gu; Jinfang Shi; Jie Xu
Journal:  J Clin Diagn Res       Date:  2015-05-01

4.  Application of a Chimeric Protein Construct having Enterotoxin B and Toxic Shock Syndrome Toxin Domains of S. aureus in Immunodiagnostics.

Authors:  R Shylaja; Devi Kalyan Kumar Thakasi; H S Murali; K Prakash Narayana Reddy; H V Batra
Journal:  Indian J Microbiol       Date:  2012-03-31       Impact factor: 2.461

5.  Genotyping of methicillin-resistant Staphylococcus aureus strains from two hospitals in Bangalore, South India.

Authors:  Gayathri Arakere; Savitha Nadig; Göte Swedberg; Ragini Macaden; Satish K Amarnath; Dasarathy Raghunath
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

6.  Multiple cases of familial transmission of community-acquired methicillin-resistant Staphylococcus aureus.

Authors:  X W Huijsdens; M G van Santen-Verheuvel; E Spalburg; M E O C Heck; G N Pluister; B A Eijkelkamp; A J de Neeling; W J B Wannet
Journal:  J Clin Microbiol       Date:  2006-08       Impact factor: 5.948

7.  Epidemiology of European community-associated methicillin-resistant Staphylococcus aureus clonal complex 80 type IV strains isolated in Denmark from 1993 to 2004.

Authors:  A R Larsen; S Böcher; M Stegger; R Goering; L V Pallesen; R Skov
Journal:  J Clin Microbiol       Date:  2007-11-07       Impact factor: 5.948

8.  Sensitive and specific detection of staphylococcal epidermolysins A and B in broth cultures by flow cytometry-assisted multiplex immunoassay.

Authors:  Olivier Joubert; Daniel Keller; Anne Pinck; Henri Monteil; Gilles Prévost
Journal:  J Clin Microbiol       Date:  2005-03       Impact factor: 5.948

9.  Antimicrobial resistance of old and recent Staphylococcus aureus isolates from poultry: first detection of livestock-associated methicillin-resistant strain ST398.

Authors:  Mostafa Nemati; Katleen Hermans; Urszula Lipinska; Olivier Denis; Ariane Deplano; Marc Struelens; Luc A Devriese; Frank Pasmans; Freddy Haesebrouck
Journal:  Antimicrob Agents Chemother       Date:  2008-07-28       Impact factor: 5.191

10.  Familial furunculosis associated with community-acquired leukocidin-positive methicillin-susceptible Staphylococcus aureus ST152.

Authors:  E Pérez-Roth; J Alcoba-Flórez; C López-Aguilar; I Gutiérrez-González; B Rivero-Pérez; S Méndez-Alvarez
Journal:  J Clin Microbiol       Date:  2009-11-11       Impact factor: 5.948
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