Literature DB >> 15297552

Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates.

Astrid Løvseth1, Semir Loncarevic, Knut G Berdal.   

Abstract

A modified multiplex PCR method for detection of nine Staphylococcus aureus enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, and sej) and one form of immunoreactive toxic shock syndrome toxin based on a previously published method (S. R. Monday and G. A. Bohach, J. Clin. Microbiol. 37:3411-3414, 1999) has been developed. The modified PCR protocol seems robust and gives reliable results.

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Year:  2004        PMID： 15297552      PMCID： PMC497657          DOI： 10.1128/JCM.42.8.3869-3872.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance.

Authors:  M Mehrotra; G Wang; W M Johnson
Journal:  J Clin Microbiol       Date:  2000-03       Impact factor: 5.948

2.  Characterization and expression analysis of Staphylococcus aureus pathogenicity island 3. Implications for the evolution of staphylococcal pathogenicity islands.

Authors:  Jeremy M Yarwood; John K McCormick; Michael L Paustian; Paul M Orwin; Vivek Kapur; Patrick M Schlievert
Journal:  J Biol Chem       Date:  2002-01-30       Impact factor: 5.157

3.  Characterization of Staphylococcus aureus enterotoxin L.

Authors:  Paul M Orwin; J Ross Fitzgerald; Donald Y M Leung; Juan A Gutierrez; Gregory A Bohach; Patrick M Schlievert
Journal:  Infect Immun       Date:  2003-05       Impact factor: 3.441

4.  Detection of seg, seh, and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S. aureus isolates Harboring seg, seh, or sei genes.

Authors:  Katsuhiko Omoe; Machiko Ishikawa; Yu Shimoda; Dong-Liang Hu; Shigeko Ueda; Kunihiro Shinagawa
Journal:  J Clin Microbiol       Date:  2002-03       Impact factor: 5.948

5.  Whole genome sequencing of meticillin-resistant Staphylococcus aureus.

Authors:  M Kuroda; T Ohta; I Uchiyama; T Baba; H Yuzawa; I Kobayashi; L Cui; A Oguchi; K Aoki; Y Nagai; J Lian; T Ito; M Kanamori; H Matsumaru; A Maruyama; H Murakami; A Hosoyama; Y Mizutani-Ui; N K Takahashi; T Sawano; R Inoue; C Kaito; K Sekimizu; H Hirakawa; S Kuhara; S Goto; J Yabuzaki; M Kanehisa; A Yamashita; K Oshima; K Furuya; C Yoshino; T Shiba; M Hattori; N Ogasawara; H Hayashi; K Hiramatsu
Journal:  Lancet       Date:  2001-04-21       Impact factor: 79.321

6.  Toxin genes and other characteristics of Staphylococcus aureus isolates from milk of cows with mastitis.

Authors:  C Annemüller; A A Hassan; C Lämmler; W Wolter; M Zschöck
Journal:  Clin Diagn Lab Immunol       Date:  2001-09

7.  Biochemical and biological properties of Staphylococcal enterotoxin K.

Authors:  P M Orwin; D Y Leung; H L Donahue; R P Novick; P M Schlievert
Journal:  Infect Immun       Date:  2001-01       Impact factor: 3.441

8.  egc, a highly prevalent operon of enterotoxin gene, forms a putative nursery of superantigens in Staphylococcus aureus.

Authors:  S Jarraud; M A Peyrat; A Lim; A Tristan; M Bes; C Mougel; J Etienne; F Vandenesch; M Bonneville; G Lina
Journal:  J Immunol       Date:  2001-01-01       Impact factor: 5.422

9.  Use of multiplex PCR to detect classical and newly described pyrogenic toxin genes in staphylococcal isolates.

Authors:  S R Monday; G A Bohach
Journal:  J Clin Microbiol       Date:  1999-10       Impact factor: 5.948

10.  High prevalence of superantigens associated with the egc locus in Staphylococcus aureus isolates from patients with atopic eczema.

Authors:  M Mempel; G Lina; M Hojka; C Schnopp; H-P Seidl; T Schäfer; J Ring; F Vandenesch; D Abeck
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2003-05-13       Impact factor: 3.267
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  31 in total

1.  Detection of stapylococcal enterotoxin, methicillin-resistant and Panton-Valentine leukocidin genes in coagulase-negative staphylococci isolated from cows and ewes with subclinical mastitis.

Authors:  Nilgün Unal; Oya Doğu Cinar
Journal:  Trop Anim Health Prod       Date:  2011-12-11       Impact factor: 1.559

2.  Isolation and characterization of a virulent bacteriophage SPW specific for Staphylococcus aureus isolated from bovine mastitis of lactating dairy cattle.

Authors:  Longping Li; Zhiying Zhang
Journal:  Mol Biol Rep       Date:  2014-07-01       Impact factor: 2.316

3.  Characterisation of mecA gene negative Staphylococcus aureus isolated from bovine mastitis milk from Northern Germany.

Authors:  O H Sheet; N T Grabowski; G Klein; F Reich; A Abdulmawjood
Journal:  Folia Microbiol (Praha)       Date:  2019-03-19       Impact factor: 2.099

4.  A probe-based method for confirmation of methicillin-resistant Staphylococcus aureus and detection of Panton-Valentine leukocidin and tst virulence genes.

Authors:  Ashok Srinivasan; Matthew J Bankowski; Steven E Seifried; Sadao Jinno; Rosalie Perkins; Seema Singh; Claire Ying; Alan D Tice; Wesley Kim; Randall T Hayden
Journal:  Diagn Microbiol Infect Dis       Date:  2011-06-11       Impact factor: 2.803

5.  Genetic variation among Staphylococcus aureus strains from Norwegian bulk milk.

Authors:  H J Jørgensen; T Mørk; D A Caugant; A Kearns; L M Rørvik
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

6.  Panton-Valentine leukocidin and some exotoxins of Staphylococcus aureus and antimicrobial susceptibility profiles of staphylococci isolated from milks of small ruminants.

Authors:  Nilgün Ünal; Şinasi Askar; Hasan Ceyhun Macun; Fatma Sakarya; Belgin Altun; Murat Yıldırım
Journal:  Trop Anim Health Prod       Date:  2011-07-29       Impact factor: 1.559

7.  Antibiotic resistance and pathogenicity factors in Staphylococcus aureus isolated from mastitic Sahiwal cattle.

Authors:  Ravinder Kumar; B R Yadav; R S Singh
Journal:  J Biosci       Date:  2011-03       Impact factor: 1.826

8.  Genetic determinants of antibiotic resistance in Staphylococcus aureus isolates from milk of mastitic crossbred cattle.

Authors:  Ravinder Kumar; B R Yadav; R S Singh
Journal:  Curr Microbiol       Date:  2009-12-03       Impact factor: 2.188

9.  Validation of virulence and epidemiology DNA microarray for identification and characterization of Staphylococcus aureus isolates.

Authors:  Richard P Spence; Victoria Wright; Dlawer A A Ala-Aldeen; David P Turner; Karl G Wooldridge; Richard James
Journal:  J Clin Microbiol       Date:  2008-02-20       Impact factor: 5.948

10.  Pulsed-field gel electrophoresis of Staphylococcus aureus isolates from atopic patients revealing presence of similar strains in isolates from children and their parents.

Authors:  Sonja Bonness; Christiane Szekat; Natalija Novak; Gabriele Bierbaum
Journal:  J Clin Microbiol       Date:  2007-12-12       Impact factor: 5.948
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