Literature DB >> 10691203

Evaluation of a rapid immunochromatographic assay for the detection of Legionella antigen in urine samples.

J Domínguez1, N Galí, L Matas, P Pedroso, A Hernández, E Padilla, V Ausina.   

Abstract

A new immunochromatographic membrane assay for detecting Legionella pneumophila serogroup 1 antigen in urine samples (Binax Now Legionella Urinary Antigen Test; Binax, USA) was evaluated. Its sensitivity, specificity and level of agreement with the Binax enzyme immunoassay were compared using nonconcentrated and concentrated urine samples. The overall agreement between the two tests was 98.1%; the specificity of both was 100%. The sensitivity of the immunochromatographic assay was 55.5% in nonconcentrated urine and 97.2% in concentrated urine in comparison with the enzyme immunoassay, using concentrated urine as the reference test. This immunochromatographic assay screens successfully for Legionella pneumophila serogroup 1 soluble antigen in concentrated urine samples.

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Year:  1999        PMID： 10691203     DOI： 10.1007/s100960050427

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  19 in total

1.  Comparison of diagnostic sensitivities of three assays (Bartels enzyme immunoassay [EIA], Biotest EIA, and Binax NOW immunochromatographic test) for detection of Legionella pneumophila serogroup 1 antigen in urine.

Authors:  Carmen Guerrero; Carmen M Toldos; Genoveva Yagüe; Cristobal Ramírez; Tomás Rodríguez; Manuel Segovia
Journal:  J Clin Microbiol       Date:  2004-01       Impact factor: 5.948

Review 2.  Immunological methods for detection and identification of infectious disease and biological warfare agents.

Authors:  Anne Harwood Peruski; Leonard F Peruski
Journal:  Clin Diagn Lab Immunol       Date:  2003-07

3.  Sensitivity of three urinary antigen tests associated with clinical severity in a large outbreak of Legionnaires' disease in The Netherlands.

Authors:  Ed P F Yzerman; Jeroen W den Boer; Kamilla D Lettinga; Joop Schellekens; Jacob Dankert; Marcel Peeters
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

4.  Centrifugal ultrafiltration method for rapid concentration of Legionella pneumophila urinary antigen.

Authors:  S Blanco; C Prat; M A Pallarés; L Matas; J Domínguez
Journal:  J Clin Microbiol       Date:  2004-09       Impact factor: 5.948

Review 5.  Optimal sampling sites and methods for detection of pathogens possibly causing community-acquired lower respiratory tract infections.

Authors:  K Loens; L Van Heirstraeten; S Malhotra-Kumar; H Goossens; M Ieven
Journal:  J Clin Microbiol       Date:  2008-11-19       Impact factor: 5.948

6.  Detection of Legionella antigen in nonconcentrated and concentrated urine samples by a new immunochromatographic assay.

Authors:  S Blanco; A Lacoma; C Prat; M A Cuesta; L Fuenzalida; I Latorre; J Domínguez
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-06-27       Impact factor: 3.267

Review 7.  Current and emerging Legionella diagnostics for laboratory and outbreak investigations.

Authors:  Jeffrey W Mercante; Jonas M Winchell
Journal:  Clin Microbiol Rev       Date:  2015-01       Impact factor: 26.132

8.  Evaluation of two new immunochromatographic assays (Rapid U Legionella antigen test and SD Bioline Legionella antigen test) for detection of Legionella pneumophila serogroup 1 antigen in urine.

Authors:  Bram M W Diederen; Marcel F Peeters
Journal:  J Clin Microbiol       Date:  2006-08       Impact factor: 5.948

9.  Clinical utility of a Legionella pneumophila urinary antigen test in a large university teaching hospital.

Authors:  Michel Dionne; Todd Hatchette; Kevin Forward
Journal:  Can J Infect Dis       Date:  2003-03

10.  Legionnaires' disease at a Dutch flower show: prognostic factors and impact of therapy.

Authors:  Kamilla D Lettinga; Annelies Verbon; Gerrit-Jan Weverling; Joop F P Schellekens; Jeroen W Den Boer; Ed P F Yzerman; Jacobus Prins; Wim G Boersma; Ruud J van Ketel; Jan M Prins; Peter Speelman
Journal:  Emerg Infect Dis       Date:  2002-12       Impact factor: 6.883
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