BACKGROUND AND OBJECTIVES: To study Haemophilus ducreyi pathogenesis, the authors developed an experimental model of infection in human volunteers. The authors analyze their cumulative experience with strain 35000 in the model and calculate the papule and pustule formation rates for estimated delivered doses (EDDs) ranging from 1 cfu to 100 cfu. STUDY DESIGN: Sixty-five volunteers were included in the analysis. A total of 139 sites were available for calculation of the papule formation rate, and 117 sites were available for calculation of the pustule formation rates. RESULTS: The effect of EDDs and probabilities of papule formation and the pustule formation were dose-dependent. Increasing the EDD resulted in a higher probability of papule and pustule formation. CONCLUSION: H ducreyi is highly infectious for humans. Inoculation of an EDD of 1 cfu causes a papule formation rate of 50%. Pustule formation rates are approximately 50% for 27 cfu and 90% for 100 cfu.
BACKGROUND AND OBJECTIVES: To study Haemophilus ducreyi pathogenesis, the authors developed an experimental model of infection in human volunteers. The authors analyze their cumulative experience with strain 35000 in the model and calculate the papule and pustule formation rates for estimated delivered doses (EDDs) ranging from 1 cfu to 100 cfu. STUDY DESIGN: Sixty-five volunteers were included in the analysis. A total of 139 sites were available for calculation of the papule formation rate, and 117 sites were available for calculation of the pustule formation rates. RESULTS: The effect of EDDs and probabilities of papule formation and the pustule formation were dose-dependent. Increasing the EDD resulted in a higher probability of papule and pustule formation. CONCLUSION: H ducreyi is highly infectious for humans. Inoculation of an EDD of 1 cfu causes a papule formation rate of 50%. Pustule formation rates are approximately 50% for 27 cfu and 90% for 100 cfu.
Authors: Stanley M Spinola; Cliffton T H Bong; Andrew L Faber; Kate R Fortney; Stacy L Bennett; Carisa A Townsend; Beth E Zwickl; Steven D Billings; Tricia L Humphreys; Margaret E Bauer; Barry P Katz Journal: Infect Immun Date: 2003-11 Impact factor: 3.441
Authors: Sherri D Rinker; Xiaoping Gu; Kate R Fortney; Beth W Zwickl; Barry P Katz; Diane M Janowicz; Stanley M Spinola; Margaret E Bauer Journal: J Infect Dis Date: 2012-08-28 Impact factor: 5.226
Authors: R S Young; K Fortney; J C Haley; A F Hood; A A Campagnari; J Wang; J A Bozue; R S Munson; S M Spinola Journal: Infect Immun Date: 1999-12 Impact factor: 3.441
Authors: Diane M Janowicz; Kate R Fortney; Barry P Katz; Jo L Latimer; Kaiping Deng; Eric J Hansen; Stanley M Spinola Journal: Infect Immun Date: 2004-08 Impact factor: 3.441
Authors: Stanley M Spinola; Kate R Fortney; Barry P Katz; Jo L Latimer; Jason R Mock; Merja Vakevainen; Eric J Hansen Journal: Infect Immun Date: 2003-12 Impact factor: 3.441