Literature DB >> 10672030

Purification, characterization and structural analysis of an abundant beta-1,3-glucanase from banana fruit.

W J Peumans1, A Barre, V Derycke, P Rougé, W Zhang, G D May, J A Delcour, F Van Leuven, E J Van Damme.   

Abstract

An abundant, catalytically active beta-1,3-endoglucanase (EC 3.2.1. 39) has been isolated from the pulp of ripe bananas. Biochemical analysis of the purified protein, molecular modelling, and molecular cloning of the corresponding gene indicate that this banana enzyme closely resembles previously characterized plant beta-glucanases with respect to its amino-acid sequence, structure and biological activity. The results described in this paper demonstrate both the occurrence of an abundant active beta-1,3-endoglucanases in fruits and also readdress the question of the possible involvement of these enzymes in the ripening and/or softening process.

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Year:  2000        PMID: 10672030     DOI: 10.1046/j.1432-1327.2000.01117.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN: 0014-2956


  12 in total

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9.  Abscisic acid stimulated ripening and gene expression in berry skins of the Cabernet Sauvignon grape.

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10.  Expression and in situ localization of two major PR proteins of grapevine berries during development and after UV-C exposition.

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