| Literature DB >> 10665536 |
R Nielsen1, J F van den Bosch, T Plambeck, V Sørensen, J P Nielsen.
Abstract
The reference strains of the 12 serotypes of Actinobacillus pleuropneumoniae express one or two of three different RTX exotoxins designated Apx I, Apx II and Apx III. The toxins are important virulence factors. In the present study, ELISAs with purified Apx I, Apx II and Apx III, respectively, as antigen were evaluated as candidates for serological diagnosis of Actinobacillus pleuropneumoniae infection in pigs. The pigs were inoculated with biotype 1, serotypes 1-12, and biotype 2, serotype 14, respectively. A strong humoral antibody response was seen to all the three antigens in most pigs irrespective of the serotype used for inoculation. However, titers to the exotoxins secreted by the serotype used for inoculation were generally highest. The results show that toxin proteins of Actinobacillus pleuropneumoniae are antigenically related and that a correlation between serotype and secretion of exotoxin is not revealed serologically in the ELISA test.Entities:
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Year: 2000 PMID： 10665536 DOI： 10.1016/s0378-1135(99)00157-1
Source DB: PubMed Journal: Vet Microbiol ISSN： 0378-1135 Impact factor: 3.293