| Literature DB >> 10660070 |
D Jakimowicz1, J Majka, B Lis, G Konopa, G Wegrzyn, W Messer, H Schrempf, J Zakrzewska-Czerwinska.
Abstract
The regulatory region of the Streptomyces dnaA gene comprises a single promoter and two DnaA boxes that are located upstream of the promoter. Comparative analysis of the dnaA promoter region from S. chrysomallus, S. lividans and S. reticuli revealed that the location, spacing and orientation of the DnaA boxes are conserved. In vitro studies demonstrated that efficient binding of the Streptomyces DnaA protein to DNA requires the presence of two DnaA boxes. In vivo analysis of dnaA promoter mutants deleted for one or both DnaA boxes indicated that the dnaA gene is autoregulated. However, the degree of derepression observed is relatively modest.Entities:
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Year: 2000 PMID： 10660070 DOI： 10.1007/pl00008652
Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925