Literature DB >> 10655405

PCR-Based assay for discrimination between invasive and contaminating Staphylococcus epidermidis strains.

N B Frebourg1, S Lefebvre, S Baert, J F Lemeland.   

Abstract

The discrimination between Staphylococcus epidermidis strains that contaminate and infect blood cultures is a daily challenge for clinical laboratories. The results of PCR detection of putative virulence genes were compared for contaminating strains, sepsis-related strains, catheter strains, and saprophytic strains. Multiplex PCR was used to explore the atlE gene, which is involved in initial adherence, the intercellular adhesion gene cluster (ica), which mediates the formation of the biofilm, and the agrA, sarA, and mecA genes, which might contribute to the pathogenicity of S. epidermidis. Whereas the atlE, agrA, and sarA genes were almost ubiquitously amplified, the ica and mecA genes were detected significantly more in infecting strains than in contaminating strains (P </= 0.02) and thus appeared to be related to the potential virulence of S. epidermidis.

Entities:  

Mesh：

Substances：

Year:  2000        PMID： 10655405      PMCID： PMC86232     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  The agent of bacillary angiomatosis. An approach to the identification of uncultured pathogens.

Authors:  D A Relman; J S Loutit; T M Schmidt; S Falkow; L S Tompkins
Journal:  N Engl J Med       Date:  1990-12-06       Impact factor: 91.245

2.  Detection of the intercellular adhesion gene cluster (ica) and phase variation in Staphylococcus epidermidis blood culture strains and mucosal isolates.

Authors:  W Ziebuhr; C Heilmann; F Götz; P Meyer; K Wilms; E Straube; J Hacker
Journal:  Infect Immun       Date:  1997-03       Impact factor: 3.441

3.  Characterization of the sar locus and its interaction with agr in Staphylococcus aureus.

Authors:  J H Heinrichs; M G Bayer; A L Cheung
Journal:  J Bacteriol       Date:  1996-01       Impact factor: 3.490

4.  Characterization of Tn917 insertion mutants of Staphylococcus epidermidis affected in biofilm formation.

Authors:  C Heilmann; C Gerke; F Perdreau-Remington; F Götz
Journal:  Infect Immun       Date:  1996-01       Impact factor: 3.441

5.  Lack of mecA transcription in slime-negative phase variants of methicillin-resistant Staphylococcus epidermidis.

Authors:  M Mempel; H Feucht; W Ziebuhr; M Endres; R Laufs; L Grüter
Journal:  Antimicrob Agents Chemother       Date:  1994-06       Impact factor: 5.191

6.  Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis.

Authors:  C Heilmann; O Schweitzer; C Gerke; N Vanittanakom; D Mack; F Götz
Journal:  Mol Microbiol       Date:  1996-06       Impact factor: 3.501

7.  Agr-related sequences in Staphylococcus lugdunensis.

Authors:  F Vandenesch; S J Projan; B Kreiswirth; J Etienne; R P Novick
Journal:  FEMS Microbiol Lett       Date:  1993-07-15       Impact factor: 2.742

8.  Association of coagulase-negative staphylococcal slime production and adherence with the development and outcome of adult septicemias.

Authors:  P Kotilainen
Journal:  J Clin Microbiol       Date:  1990-12       Impact factor: 5.948

9.  Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory.

Authors:  D J Geha; J R Uhl; C A Gustaferro; D H Persing
Journal:  J Clin Microbiol       Date:  1994-07       Impact factor: 5.948

Review 10.  Update on clinical significance of coagulase-negative staphylococci.

Authors:  W E Kloos; T L Bannerman
Journal:  Clin Microbiol Rev       Date:  1994-01       Impact factor: 26.132
View more
  67 in total

1.  Detection of Staphylococcus aureus Clinical Isolates Harboring the ica Gene Cluster Needed for Biofilm Establishment.

Authors:  Juana Victoria Martín-López; Eduardo Pérez-Roth; Félix Claverie-Martín; Oscar Díez Gil; Ninivé Batista; Manuel Morales; Sebastián Méndez-Alvarez
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

2.  Involvement of adherence and adhesion Staphylococcus epidermidis genes in pacemaker lead-associated infections.

Authors:  Didier Klug; Frédéric Wallet; Salem Kacet; René J Courcol
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

3.  Biofilm formation and the presence of the intercellular adhesion locus ica among staphylococci from food and food processing environments.

Authors:  Trond Møretrø; Lene Hermansen; Askild L Holck; Maan S Sidhu; Knut Rudi; Solveig Langsrud
Journal:  Appl Environ Microbiol       Date:  2003-09       Impact factor: 4.792

4.  icaR encodes a transcriptional repressor involved in environmental regulation of ica operon expression and biofilm formation in Staphylococcus epidermidis.

Authors:  Kevin M Conlon; Hilary Humphreys; James P O'Gara
Journal:  J Bacteriol       Date:  2002-08       Impact factor: 3.490

5.  mecA is not involved in the sigmaB-dependent switch of the expression phenotype of methicillin resistance in Staphylococcus epidermidis.

Authors:  Johannes K-M Knobloch; Sebastian Jäger; Jörn Huck; Matthias A Horstkotte; Dietrich Mack
Journal:  Antimicrob Agents Chemother       Date:  2005-03       Impact factor: 5.191

6.  Polymorphism of the cell wall-anchoring domain of the autolysin-adhesin AtlE and its relationship to sequence type, as revealed by multilocus sequence typing of invasive and commensal Staphylococcus epidermidis strains.

Authors:  V Sivadon; M Rottman; J-C Quincampoix; E Prunier; P de Mazancourt; L Bernard; A Lortat-Jacob; P Piriou; T Judet; J-L Gaillard
Journal:  J Clin Microbiol       Date:  2006-05       Impact factor: 5.948

7.  Analysis of different genetic traits and their association with biofilm formation in Staphylococcus epidermidis isolates from central venous catheter infections.

Authors:  D Petrelli; C Zampaloni; S D'Ercole; M Prenna; P Ballarini; S Ripa; L A Vitali
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2006-12       Impact factor: 3.267

8.  Strong slime production is a marker of clinical significance in Staphylococcus epidermidis isolated from intravascular catheters.

Authors:  M Mateo; J R Maestre; L Aguilar; M J Giménez; J J Granizo; J Prieto
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2007-12-18       Impact factor: 3.267

9.  The biofilm-positive Staphylococcus epidermidis isolates in raw materials, foodstuffs and on contact surfaces in processing plants.

Authors:  J Schlegelová; V Babák; M Holasová; M Dendis
Journal:  Folia Microbiol (Praha)       Date:  2009-04-18       Impact factor: 2.099

10.  New structure of phage-related islands carrying fusB and a virulence gene in fusidic acid-resistant Staphylococcus epidermidis.

Authors:  Hsiao-Jan Chen; Ya-Chun Chang; Jui-Chang Tsai; Wei-Chun Hung; Yu-Tzu Lin; Shang-Jie You; Sung-Pin Tseng; Lee-Jene Teng
Journal:  Antimicrob Agents Chemother       Date:  2013-08-26       Impact factor: 5.191
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.