Literature DB >> 10648814

Functional expression of His-tagged sensory rhodopsin I in Escherichia coli.

G Schmies1, I Chizhov, M Engelhard.   

Abstract

Sensory rhodopsin I (SRI) from Halobacterium salinarum was functionally expressed in Escherichia coli and subsequently purified to homogeneity using a C-terminal His-tag anchor. Yields of 3-4 mg SRI/l cell culture can be obtained. The absorption and photocycle properties of SRI were similar if not indistinguishable from those of the homologously expressed SRI. A global fit analysis of the photocycle data and the calculation of the spectra of states provided strong evidence for the existence of an N-like intermediate.

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Year:  2000        PMID： 10648814     DOI： 10.1016/s0014-5793(99)01760-3

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  6 in total

1.  Multicolored protein conformation states in the photocycle of transducer-free sensory rhodopsin-I.

Authors:  I Szundi; T E Swartz; R A Bogomolni
Journal:  Biophys J       Date:  2001-01       Impact factor: 4.033

2.  A novel six-rhodopsin system in a single archaeon.

Authors:  Hsu-Yuan Fu; Yu-Cheng Lin; Yung-Ning Chang; Hsiaochu Tseng; Ching-Che Huang; Kang-Cheng Liu; Ching-Shin Huang; Che-Wei Su; Rueyhung Roc Weng; Yin-Yu Lee; Wailap Victor Ng; Chii-Shen Yang
Journal:  J Bacteriol       Date:  2010-08-27       Impact factor: 3.490

Review 3.  Molecular Biology of Microbial Rhodopsins.

Authors:  Martin Engelhard
Journal:  Methods Mol Biol       Date:  2022

4.  Electrophysiological characterization of specific interactions between bacterial sensory rhodopsins and their transducers.

Authors:  G Schmies; M Engelhard; P G Wood; G Nagel; E Bamberg
Journal:  Proc Natl Acad Sci U S A       Date:  2001-01-30       Impact factor: 11.205

5.  Monitoring the Progression of Cell-Free Expression of Microbial Rhodopsins by Surface Enhanced IR Spectroscopy: Resolving a Branch Point for Successful/Unsuccessful Folding.

Authors:  Kenichi Ataka; Axel Baumann; Jheng-Liang Chen; Aoife Redlich; Joachim Heberle; Ramona Schlesinger
Journal:  Front Mol Biosci       Date:  2022-07-14

6.  Using Haloarcula marismortui bacteriorhodopsin as a fusion tag for enhancing and visible expression of integral membrane proteins in Escherichia coli.

Authors:  Min-Feng Hsu; Tsung-Fu Yu; Chia-Cheng Chou; Hsu-Yuan Fu; Chii-Shen Yang; Andrew H J Wang
Journal:  PLoS One       Date:  2013-02-15       Impact factor: 3.240
  6 in total

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