Literature DB >> 1064875

Mapping of adenovirus messenger RNA by electron microscopy.

H Westphal, J Meyer, J V Maizel.   

Abstract

Late adenovirus messenger RNA was annealed to complementary regions of partially melted viral double-stranded DNA. The RNA. DNA hybrid regions within the DNA molecules were visualized as loops in the electron microscope. Loops occurred at several regions of the DNA, most frequently, however, at a location near the center of the molecule. This hybridization technique appears well suited for an accurate mapping of messenger RNA, as well as for studies of RNA processing.

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Year:  1976        PMID： 1064875      PMCID： PMC430450          DOI： 10.1073/pnas.73.6.2069

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  14 in total

1.  Ribonuclease 3 does not degrade deoxyribonucleic acid-ribonucleic acid hybrids.

Authors:  R J Crouch
Journal:  J Biol Chem       Date:  1974-02-25       Impact factor: 5.157

2.  Electrophoresis of RNA in formamide.

Authors:  J C Pinder; D Z Staynov; W B Gratzer
Journal:  Biochemistry       Date:  1974-12-17       Impact factor: 3.162

3.  Cell-free synthesis of adenovirus coat proteins.

Authors:  L Eron; R Callahan; H Westphal
Journal:  J Biol Chem       Date:  1974-10-10       Impact factor: 5.157

4.  Length measurements of RNA synthesized in vitro by Escherichia coli RNA polymerase.

Authors:  H Delius; H Westphal; N Axelrod
Journal:  J Mol Biol       Date:  1973-03-15       Impact factor: 5.469

5.  Amount of viral DNA in the genome of cells transformed by adenovirus type 2.

Authors:  U Pettersson; J Sambrook
Journal:  J Mol Biol       Date:  1973-01       Impact factor: 5.469

6.  Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication.

Authors:  L Philipson; R Wall; G Glickman; J E Darnell
Journal:  Proc Natl Acad Sci U S A       Date:  1971-11       Impact factor: 11.205

7.  Enzyme from calf thymus degrading the RNA moiety of DNA-RNA Hybrids: effect on DNA-dependent RNA polymerase.

Authors:  H Stein; P Hausen
Journal:  Science       Date:  1969-10-17       Impact factor: 47.728

8.  Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments.

Authors:  J B Lewis; J F Atkins; C W Anderson; P R Baum; R F Gesteland
Journal:  Proc Natl Acad Sci U S A       Date:  1975-04       Impact factor: 11.205

9.  Cell-free synthesis of adenovirus 2 proteins programmed by fractionated messenger RNA: a comparison of polypeptide products and messenger RNA lengths.

Authors:  C W Anderson; J B Lewis; J F Atkins; R F Gesteland
Journal:  Proc Natl Acad Sci U S A       Date:  1974-07       Impact factor: 11.205

10.  Amplified ribosomal DNA from Xenopus laevis has heterogeneous spacer lengths.

Authors:  P K Wellauer; R H Reeder; D Carroll; D D Brown; A Deutch; T Higashinakagawa; I B Dawid
Journal:  Proc Natl Acad Sci U S A       Date:  1974-07       Impact factor: 11.205
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  8 in total

1.  Crosslinked histone octamer as a model of the nucleosome core.

Authors:  A Stein; M Bina-Stein; R T Simpson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-07       Impact factor: 11.205

2.  Spliced adenovirus-associated virus RNA.

Authors:  C A Laughlin; H Westphal; B J Carter
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

3.  Loop structures in hybrids of early RNA and the separated strands of adenovirus DNA.

Authors:  G R Kitchingman; S P Lai; H Westphal
Journal:  Proc Natl Acad Sci U S A       Date:  1977-10       Impact factor: 11.205

4.  Physical organization of subgroup B human adenovirus genomes.

Authors:  C Tibbetts
Journal:  J Virol       Date:  1977-11       Impact factor: 5.103

5.  Mapping of transcription sites of simian virus 40-specific late 16S and 19S mRNA by electron microscopy.

Authors:  E May; J V Maizel; N P Salzman
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

6.  Spliced segments at the 5' terminus of adenovirus 2 late mRNA.

Authors:  S M Berget; C Moore; P A Sharp
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205

7.  Early gene expression of adenovirus type 2: R-loop mapping of mRNA and time course of viral DNA, mRNA, and protein synthesis.

Authors:  P D Neuwald; J Meyer; J V Maizel; H Westpahl
Journal:  J Virol       Date:  1977-03       Impact factor: 5.103

8.  Electron microscopy of late adenovirus type 2 mRNA hybridized to double-stranded viral DNA.

Authors:  J Meyer; P D Neuwald; S P Lai; J V Maizel; H Westphal
Journal:  J Virol       Date:  1977-03       Impact factor: 5.103
  8 in total

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