| Literature DB >> 10643912 |
E C Grisard1, M Steindel, J J Shaw, E A Ishikawa, C J Carvalho-Pinto, I Eger-Mangrich, H K Toma, J H Lima, A J Romanha, D A Campbell.
Abstract
Four Leishmania sp. samples were isolated from autochthonous human cases of American cutaneous leishmaniasis (ACL) in Santa Catarina State, southern Brazil. These strains were characterized using indirect immunofluorescence with a panel of Leishmania-specific monoclonal antibodies (MAbs), and by PCR amplification and hybridization assay of the mini-exon gene with group specific probes. The results obtained with the MAbs were in agreement with the genetic marker. Two isolates (MHOM/BR/89/JSC89-H1 and MHOM/BR/89/JSC89-H2) were identified as L. (Leishmania) amazonensis and two (MHOM/BR/96/LSC96-H3 and MHOM/BR/97/LSC97-H4) as L. (Viannia) braziliensis. The southernmost autochthonous cases of ACL in Brazil are due to two different Leishmania sp. species, confirming the spreading of ACL on the American continent.Entities:
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Year: 2000 PMID: 10643912 DOI: 10.1016/s0001-706x(99)00054-6
Source DB: PubMed Journal: Acta Trop ISSN: 0001-706X Impact factor: 3.112