Mouse-chick chimera: an experimental system for study of somite development.

As the mammalian embryo is implanted in the uterus and not readily accessible to direct observation or manipulation, much of our understanding of mammalian somite development is based on findings in lower vertebrates. One means of overcoming the difficulties raised by intrauterine development is to engraft mouse tissue in ovo. The experiments described in this chapter relate to the unilateral replacement of somites in chick embryo with those from mouse fetus. Mouse somites differentiate in ovo in dermis, cartilage, and skeletal muscle and are able to migrate into chick host limb. A LacZ transgenic mouse strain was used to ascertain the role of the implanted somites in forming epaxial and hypaxial muscle in the chick embryo. Myogenesis occurred normally in in ovo developing mouse somites, and muscle cells from mouse myotome formed neuromuscular contacts with chick motor axons. After fragments of fetal mouse neural primordium were transplanted into chick embryo, mouse neural tube contributed to the mechanism maintaining myogenesis in the somites of the host embryo. A recently developed double-grafting procedure involving neural tube and somites from knockout mouse strains should elucidate the molecular events involved in early somitogenesis.