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Literature DB >> 10618292

Development of positive selectable markers for the fungal pathogen Cryptococcus neoformans.

Abstract

Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningitis in approximately 10% of patients with AIDS. New selectable markers which confer resistance to G418 or phleomycin when transformed into C. neoformans were made. A hygromycin-selectable marker was modified to allow selection with a single copy of the marker.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 2000 PMID： 10618292 PMCID： PMC95837 DOI： 10.1128/CDLI.7.1.125-128.2000

Source DB: PubMed Journal: Clin Diagn Lab Immunol ISSN： 1071-412X

6 in total

1. Genetic and biochemical studies establish that the fungicidal effect of a fully depeptidized inhibitor of Cryptococcus neoformans myristoyl-CoA:protein N-myristoyltransferase (Nmt) is Nmt-dependent.

Authors: J K Lodge; E Jackson-Machelski; M Higgins; C A McWherter; J A Sikorski; B Devadas; J I Gordon
Journal: J Biol Chem Date: 1998-05-15 Impact factor: 5.157

2. Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation.

Authors: J C Edman; K J Kwon-Chung
Journal: Mol Cell Biol Date: 1990-09 Impact factor: 4.272

3. Dominant selection system for use in Cryptococcus neoformans.

Authors: G M Cox; D L Toffaletti; J R Perfect
Journal: J Med Vet Mycol Date: 1996 Nov-Dec

4. Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA.

Authors: D L Toffaletti; T H Rude; S A Johnston; D T Durack; J R Perfect
Journal: J Bacteriol Date: 1993-03 Impact factor: 3.490

5. The Cryptococcus neoformans GAL7 gene and its use as an inducible promoter.

Authors: B L Wickes; J C Edman
Journal: Mol Microbiol Date: 1995-06 Impact factor: 3.501

6. Formation of a minichromosome in Cryptococcus neoformans as a result of electroporative transformation.

Authors: A Varma; K J Kwon-Chung
Journal: Curr Genet Date: 1994-07 Impact factor: 3.886

6 in total

48 in total

Review 1. Milestones in Candida albicans gene manipulation.

Authors: Dhanushki P Samaranayake; Steven D Hanes
Journal: Fungal Genet Biol Date: 2011-04-14 Impact factor: 3.495

2. Gene disruption in Cryptococcus neoformans and Cryptococcus gattii by in vitro transposition.

Authors: Guanggan Hu; James W Kronstad
Journal: Curr Genet Date: 2006-01-06 Impact factor: 3.886

3. Combination of amphotericin B with flucytosine is active in vitro against flucytosine-resistant isolates of Cryptococcus neoformans.

Authors: Patrick Schwarz; Guilhem Janbon; Françoise Dromer; Olivier Lortholary; Eric Dannaoui
Journal: Antimicrob Agents Chemother Date: 2006-10-16 Impact factor: 5.191

4. PKC1 is essential for protection against both oxidative and nitrosative stresses, cell integrity, and normal manifestation of virulence factors in the pathogenic fungus Cryptococcus neoformans.

Authors: Kimberly J Gerik; Sujit R Bhimireddy; Jan S Ryerse; Charles A Specht; Jennifer K Lodge
Journal: Eukaryot Cell Date: 2008-08-08

Development of positive selectable markers for the fungal pathogen Cryptococcus neoformans.

1. Genetic and biochemical studies establish that the fungicidal effect of a fully depeptidized inhibitor of Cryptococcus neoformans myristoyl-CoA:protein N-myristoyltransferase (Nmt) is Nmt-dependent.

2. Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation.

3. Dominant selection system for use in Cryptococcus neoformans.

4. Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA.

5. The Cryptococcus neoformans GAL7 gene and its use as an inducible promoter.

6. Formation of a minichromosome in Cryptococcus neoformans as a result of electroporative transformation.

Review 1. Milestones in Candida albicans gene manipulation.

2. Gene disruption in Cryptococcus neoformans and Cryptococcus gattii by in vitro transposition.

3. Combination of amphotericin B with flucytosine is active in vitro against flucytosine-resistant isolates of Cryptococcus neoformans.

4. PKC1 is essential for protection against both oxidative and nitrosative stresses, cell integrity, and normal manifestation of virulence factors in the pathogenic fungus Cryptococcus neoformans.

5. The copper regulon of the human fungal pathogen Cryptococcus neoformans H99.

6. Pbx proteins in Cryptococcus neoformans cell wall remodeling and capsule assembly.

7. Expression of hygromycin phosphotransferase alters virulence of Histoplasma capsulatum.

8. Three galactose inducible promoters for use in C. neoformans var. grubii.

9. Challenge of Drosophila melanogaster with Cryptococcus neoformans and role of the innate immune response.

10. UGE1 and UGE2 regulate the UDP-glucose/UDP-galactose equilibrium in Cryptococcus neoformans.