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Literature DB >> 18952189

Three galactose inducible promoters for use in C. neoformans var. grubii.

Jack A Ruff¹, Jennifer K Lodge, Lorina G Baker.

Abstract

Cryptococcus neoformans is the causative agent of cryptococcal meningoencephalitis, most frequently occurring in immunocompromised individuals. There are three varieties of C. neoformans, var. grubii, var. neoformans, and var. gatti. Worldwide var. grubii is the most prevalent clinical isolate. However, few tools for the study of essential genes in var. grubii exist. Here we describe three endogenous inducible promoters for use in the study of this important opportunistic pathogen. We identified eight potential homologs of S. cerevisiae galactose genes in var. grubii. We found that GAL1, GAL7, and UGE2 were regulated by glucose and galactose and can be used successfully during mating. Our analysis indicated these promoters should prove to be excellent tools for analysis of genes in var. grubii.

Entities: Chemical Disease Gene Species

Mesh：

Substances：
Galactose

Year: 2008 PMID： 18952189 PMCID： PMC2654232 DOI： 10.1016/j.fgb.2008.10.003

Source DB: PubMed Journal: Fungal Genet Biol ISSN： 1087-1845 Impact factor: 3.495

32 in total

1. Genetic and biochemical studies establish that the fungicidal effect of a fully depeptidized inhibitor of Cryptococcus neoformans myristoyl-CoA:protein N-myristoyltransferase (Nmt) is Nmt-dependent.

Authors: J K Lodge; E Jackson-Machelski; M Higgins; C A McWherter; J A Sikorski; B Devadas; J I Gordon
Journal: J Biol Chem Date: 1998-05-15 Impact factor: 5.157

2. GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae.

Authors: J F Tschopp; S D Emr; C Field; R Schekman
Journal: J Bacteriol Date: 1986-04 Impact factor: 3.490

3. Simplified isolation of chromosomal and plasmid DNA from yeasts.

Authors: H Fujimura; Y Sakuma
Journal: Biotechniques Date: 1993-04 Impact factor: 1.993

4. Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation.

Authors: J C Edman; K J Kwon-Chung
Journal: Mol Cell Biol Date: 1990-09 Impact factor: 4.272

5. Genetic and molecular analysis of the GAL3 gene in the expression of the galactose/melibiose regulon of Saccharomyces cerevisiae.

Authors: T E Torchia; J E Hopper
Journal: Genetics Date: 1986-06 Impact factor: 4.562

6. Transcription of a yeast phosphoglucomutase isozyme gene is galactose inducible and glucose repressible.

Authors: D Oh; J E Hopper
Journal: Mol Cell Biol Date: 1990-04 Impact factor: 4.272

7. The gene encoding phosphoribosylaminoimidazole carboxylase (ADE2) is essential for growth of Cryptococcus neoformans in cerebrospinal fluid.

Authors: J R Perfect; D L Toffaletti; T H Rude
Journal: Infect Immun Date: 1993-10 Impact factor: 3.441

8. Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA.

Authors: D L Toffaletti; T H Rude; S A Johnston; D T Durack; J R Perfect
Journal: J Bacteriol Date: 1993-03 Impact factor: 3.490

9. Targeted gene replacement demonstrates that myristoyl-CoA: protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans.

Authors: J K Lodge; E Jackson-Machelski; D L Toffaletti; J R Perfect; J I Gordon
Journal: Proc Natl Acad Sci U S A Date: 1994-12-06 Impact factor: 11.205

10. The Cryptococcus neoformans GAL7 gene and its use as an inducible promoter.

Authors: B L Wickes; J C Edman
Journal: Mol Microbiol Date: 1995-06 Impact factor: 3.501

17 in total

1. Sex-induced silencing defends the genome of Cryptococcus neoformans via RNAi.

Authors: Xuying Wang; Yen-Ping Hsueh; Wenjun Li; Anna Floyd; Rebecca Skalsky; Joseph Heitman
Journal: Genes Dev Date: 2010-11-15 Impact factor: 11.361

2. A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome.

Authors: Rajendra Upadhya; Woei C Lam; Brian T Maybruck; Maureen J Donlin; Andrew L Chang; Sarah Kayode; Kate L Ormerod; James A Fraser; Tamara L Doering; Jennifer K Lodge
Journal: Fungal Genet Biol Date: 2017-09-12 Impact factor: 3.495

Three galactose inducible promoters for use in C. neoformans var. grubii.

1. Genetic and biochemical studies establish that the fungicidal effect of a fully depeptidized inhibitor of Cryptococcus neoformans myristoyl-CoA:protein N-myristoyltransferase (Nmt) is Nmt-dependent.

2. GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae.

3. Simplified isolation of chromosomal and plasmid DNA from yeasts.

4. Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation.

5. Genetic and molecular analysis of the GAL3 gene in the expression of the galactose/melibiose regulon of Saccharomyces cerevisiae.

6. Transcription of a yeast phosphoglucomutase isozyme gene is galactose inducible and glucose repressible.

7. The gene encoding phosphoribosylaminoimidazole carboxylase (ADE2) is essential for growth of Cryptococcus neoformans in cerebrospinal fluid.

8. Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA.

9. Targeted gene replacement demonstrates that myristoyl-CoA: protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans.

10. The Cryptococcus neoformans GAL7 gene and its use as an inducible promoter.

1. Sex-induced silencing defends the genome of Cryptococcus neoformans via RNAi.

2. A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome.

3. Isolation of conditional mutations in genes essential for viability of Cryptococcus neoformans.

Review 4. New technology and resources for cryptococcal research.

5. RNA interference in Cryptococcus neoformans.

6. Genetic analysis of Hsp90 function in Cryptococcus neoformans highlights key roles in stress tolerance and virulence.

7. Going green in Cryptococcus neoformans: the recycling of a selectable drug marker.

8. Chitinases are essential for sexual development but not vegetative growth in Cryptococcus neoformans.

9. The link between morphotype transition and virulence in Cryptococcus neoformans.

10. Conditional promoters for analysis of essential genes in Zymoseptoria tritici.