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Literature DB >> 1061111

Synthesis of acetylcholine receptors by cultured chick myotubes and denervated mouse extensor digitorum longus muscles.

Abstract

Mono-[125I]iodo- alpha - bungarotoxin - receptor complexes extracted from chick myotube cultures as well as from adult denervated extensor digitorum longus muscles of the mouse have been banded at their buoyant density in gradients of metrizamide-deuterium oxide. When cultures or denervated adult muscles are preincubated in media containing 2H- or 13C-substituted amino acids under conditions of active receptor accumulation, the mono-[125I]iodo-alpha-bungarotoxin-receptor complexes have an increased buoyant density and band in a position of higher density in the gradient relative to a marker of mono-[131I]iodo-alpha-bungarotoxin-receptor complexes extracted from cells preincubated in normal media. It is concluded that the accumulation of receptors on the surfaces of cultured chick myotubes and on the non-synaptic surfaces of extensor digitorum longus muscles following denervation are the result of de novo synthesis.

Entities: Chemical Species

Mesh：

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Year: 1976 PMID： 1061111 PMCID： PMC335860 DOI： 10.1073/pnas.73.1.161

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

15 in total

1. Junctional and extra-junctional acetylcholine receptors in skeletal muscle fibres.

Authors: R MILEDI
Journal: J Physiol Date: 1960-04 Impact factor: 5.182

2. Metrizamide, a new density-gradient medium.

Authors: D Rickwood; G D Birnie
Journal: FEBS Lett Date: 1975-02-01 Impact factor: 4.124

3. Synthesis of acetylcholine receptor by denervated rat diaphragm muscle.

Authors: J P Brockes; Z W Hall
Journal: Proc Natl Acad Sci U S A Date: 1975-04 Impact factor: 11.205

4. Development of acetylcholine sensitivity during myogenesis.

Authors: D Fambrough; J E Rash
Journal: Dev Biol Date: 1971-09 Impact factor: 3.582

5. Acetycholine receptor production and incorporation into membranes of developing muscle fibers.

Authors: H C Hartzell; D M Fambrough
Journal: Dev Biol Date: 1973-01 Impact factor: 3.582

6. Synaptic potentials recorded in cell cultures of nerve and muscle.

Authors: G D Fischbach
Journal: Science Date: 1970-09-25 Impact factor: 47.728

7. A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid.

Authors: P Filner; J E Varner
Journal: Proc Natl Acad Sci U S A Date: 1967-10 Impact factor: 11.205

8. Metrizamide in D2O--a novel solute for the separation of labeled and unlabeled proteins by equilibrium density gradient centrifugation.

Authors: A Hüttermann; U Guntermann
Journal: Anal Biochem Date: 1975-04 Impact factor: 3.365

9. Acetylcholine sensitivity of muscle fiber membranes: mechanism of regulation by motoneurons.

Authors: D M Fambrough
Journal: Science Date: 1970-04-17 Impact factor: 47.728

10. Acetylcholine receptor turnover in membranes of developing muscle fibers.

Authors: P N Devreotes; D M Fambrough
Journal: J Cell Biol Date: 1975-05 Impact factor: 10.539

10 in total

1. Light-mediated Activation of Nitrate Reductase in Synchronous Chlorella.

Authors: R Tischner
Journal: Plant Physiol Date: 1978-08 Impact factor: 8.340

2. Development of electrophysiological and biochemical membrane properties during differentiation of embryonic skeletal muscle in culture.

Authors: I Spector; J M Prives
Journal: Proc Natl Acad Sci U S A Date: 1977-11 Impact factor: 11.205

Synthesis of acetylcholine receptors by cultured chick myotubes and denervated mouse extensor digitorum longus muscles.

1. Junctional and extra-junctional acetylcholine receptors in skeletal muscle fibres.

2. Metrizamide, a new density-gradient medium.

3. Synthesis of acetylcholine receptor by denervated rat diaphragm muscle.

4. Development of acetylcholine sensitivity during myogenesis.

5. Acetycholine receptor production and incorporation into membranes of developing muscle fibers.

6. Synaptic potentials recorded in cell cultures of nerve and muscle.

7. A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid.

8. Metrizamide in D2O--a novel solute for the separation of labeled and unlabeled proteins by equilibrium density gradient centrifugation.

9. Acetylcholine sensitivity of muscle fiber membranes: mechanism of regulation by motoneurons.

10. Acetylcholine receptor turnover in membranes of developing muscle fibers.

1. Light-mediated Activation of Nitrate Reductase in Synchronous Chlorella.

2. Development of electrophysiological and biochemical membrane properties during differentiation of embryonic skeletal muscle in culture.

3. Regulation of acetylcholine receptor levels by a cholinergic agonist in mouse muscle cell cultures.

4. Inhibition of methyltransferase reduces the turnover of acetylcholine receptors.

5. The regulation of extrajunctional acetylcholine receptors in the denervated rat diaphragm muscle in culture.

6. Nerve-induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells.

7. Estrogen receptor separation employing sucrose deuterium oxide gradients generated during sample centrifugation.

8. Loss of extrasynaptic acetylcholine sensitivity upon reinnervation of parasympathetic ganglion cells.

9. Effect of myasthenic patients' immunoglobulin on acetylcholine receptor turnover: selectivity of degradation process.

10. Skeletal muscle denervation activates acetylcholine receptor genes.