Literature DB >> 10606606

Real-time quantitative PCR detection of genetically modified Maximizer maize and Roundup Ready soybean in some representative foods.

M Vaïtilingom1, H Pijnenburg, F Gendre, P Brignon.   

Abstract

A fast and quantitative method was developed to detect transgenic "Maximizer" maize "event 176" (Novartis) and "Roundup Ready" soybean (Monsanto) in food by real-time quantitative PCR. The use of the ABI Prism 7700 sequence detection system allowed the determination of the amplified product accumulation through a fluorogenic probe (TaqMan). Fluorescent dyes were chosen in such a way as to coamplify total and transgenic DNA in the same tube. Using real-time quantitative PCR, 2 pg of transgenic or total DNA per gram of starting sample was detected in 3 h after DNA extraction and the relative amounts of "Maximizer" maize and "Roundup Ready" soybean in some representative food products were quantified.

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Year:  1999        PMID： 10606606     DOI： 10.1021/jf981208v

Source DB:  PubMed          Journal:  J Agric Food Chem        ISSN： 0021-8561            Impact factor:   5.279


  14 in total

1.  A novel real-time quantitative PCR method using attached universal template probe.

Authors:  Yuanli Zhang; Dabing Zhang; Wenquan Li; Jianqun Chen; Yufa Peng; Wei Cao
Journal:  Nucleic Acids Res       Date:  2003-10-15       Impact factor: 16.971

2.  Detection of genetically modified organisms in food: comparison among three different DNA extraction methods.

Authors:  B Vodret; M Milia; M G Orani; G Serratrice; M R Mancuso
Journal:  Vet Res Commun       Date:  2007-08       Impact factor: 2.459

3.  Validation of a cotton-specific gene, Sad1, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic cottons.

Authors:  Litao Yang; Jianxiu Chen; Cheng Huang; Yuhui Liu; Shirong Jia; Liangwen Pan; Dabing Zhang
Journal:  Plant Cell Rep       Date:  2005-02-22       Impact factor: 4.570

4.  A specific real-time quantitative PCR detection system for event MON810 in maize YieldGard based on the 3'-transgene integration sequence.

Authors:  Marta Hernández; Maria Pla; Teresa Esteve; Salomé Prat; Pere Puigdomènech; Alejandro Ferrando
Journal:  Transgenic Res       Date:  2003-04       Impact factor: 2.788

5.  PCR-free quantitative detection of genetically modified organism from raw materials. An electrochemiluminescence-based bio bar code method.

Authors:  Debin Zhu; Yabing Tang; Da Xing; Wei R Chen
Journal:  Anal Chem       Date:  2008-04-03       Impact factor: 6.986

6.  A novel multiplex quantitative DNA array based PCR (MQDA-PCR) for quantification of transgenic maize in food and feed.

Authors:  Knut Rudi; Ida Rud; Askild Holck
Journal:  Nucleic Acids Res       Date:  2003-06-01       Impact factor: 16.971

7.  PCR-free detection of genetically modified organisms using magnetic capture technology and fluorescence cross-correlation spectroscopy.

Authors:  Xiaoming Zhou; Da Xing; Yonghong Tang; Wei R Chen
Journal:  PLoS One       Date:  2009-11-26       Impact factor: 3.240

Review 8.  How to deal with the upcoming challenges in GMO detection in food and feed.

Authors:  Sylvia R M Broeders; Sigrid C J De Keersmaecker; Nancy H C Roosens
Journal:  J Biomed Biotechnol       Date:  2012-10-21

9.  A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids.

Authors:  Litao Yang; Wanqi Liang; Lingxi Jiang; Wenquan Li; Wei Cao; Zoe A Wilson; Dabing Zhang
Journal:  BMC Mol Biol       Date:  2008-06-04       Impact factor: 2.946

10.  Discrimination of Transgenic Rice containing the Cry1Ab Protein using Terahertz Spectroscopy and Chemometrics.

Authors:  Wendao Xu; Lijuan Xie; Zunzhong Ye; Weilu Gao; Yang Yao; Min Chen; Jianyuan Qin; Yibin Ying
Journal:  Sci Rep       Date:  2015-07-08       Impact factor: 4.379
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