Literature DB >> 105963

Regulation of newly evolved enzymes. IV. Directed evolution of the Ebg repressor.

B G Hall.   

Abstract

In Escherichia coli, the wild-type repressor of ebg (evolved beta-galactosidase) enzyme synthesis, specified by the ebgR+ gene, responds very weakly to lactulose (fructose-beta-D-galactopyranoside). Selection for a functional repressor that responds strongly to lactulose as an inducer reveals the existence of ebgR+L mutants, which occur spontaneously at a frequency of about 2 X 10(-10) . EBGR+L mutants are pleiotropic in that they specify ebg repressor with a greatly increased response to lactulose, lactose, galactose-arabinoside and methyl-galactoside as inducers. Selection of ebgR+L mutants is discussed within the framework of directed evolution of a regulatory function.

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Year:  1978        PMID： 105963      PMCID： PMC1213912     

Source DB:  PubMed          Journal:  Genetics        ISSN： 0016-6731            Impact factor:   4.562


  5 in total

1.  EVOLUTION OF A CATABOLIC PATHWAY IN BACTERIA.

Authors:  S A LERNER; T T WU; E C LIN
Journal:  Science       Date:  1964-12-04       Impact factor: 47.728

2.  Genetic regulatory mechanisms and the ecological niche of Escherichia coli.

Authors:  M A Savageau
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205

Review 3.  The evolution of bacterial enzyme systems.

Authors:  G D Hegeman; S L Rosenberg
Journal:  Annu Rev Microbiol       Date:  1970       Impact factor: 15.500

4.  Biochemical and genetic studies with regulator mutants of the Pseudomonas aeruginosa 8602 amidase system.

Authors:  W J Brammar; P H Clarke; A J Skinner
Journal:  J Gen Microbiol       Date:  1967-04

5.  Metabolism of D-arabinose: a new pathway in Escherichia coli.

Authors:  D J LeBlanc; R P Mortlock
Journal:  J Bacteriol       Date:  1971-04       Impact factor: 3.490
  5 in total
  9 in total

Review 1.  Laboratory-directed protein evolution.

Authors:  Ling Yuan; Itzhak Kurek; James English; Robert Keenan
Journal:  Microbiol Mol Biol Rev       Date:  2005-09       Impact factor: 11.056

2.  Spectra of spontaneous growth-dependent and adaptive mutations at ebgR.

Authors:  B G Hall
Journal:  J Bacteriol       Date:  1999-02       Impact factor: 3.490

3.  On the specificity of adaptive mutations.

Authors:  B G Hall
Journal:  Genetics       Date:  1997-01       Impact factor: 4.562

4.  Adaptive mutagenesis at ebgR is regulated by PhoPQ.

Authors:  B G Hall
Journal:  J Bacteriol       Date:  1998-06       Impact factor: 3.490

5.  Adaptive mutations in Escherichia coli as a model for the multiple mutational origins of tumors.

Authors:  B G Hall
Journal:  Proc Natl Acad Sci U S A       Date:  1995-06-06       Impact factor: 11.205

6.  Topological repression of gene activity by a transposable element.

Authors:  H W Stokes; B G Hall
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

7.  The catalytic consequences of experimental evolution. Transition-state structure during catalysis by the evolved beta-galactosidases of Escherichia coli (ebg enzymes) changed by a single mutational event.

Authors:  B F Li; D Holdup; C A Morton; M L Sinnott
Journal:  Biochem J       Date:  1989-05-15       Impact factor: 3.857

8.  An evolvant of Escherichia coli that employs the L-fucose pathway also for growth on L-galactose and D-arabinose.

Authors:  Y Zhu; E C Lin
Journal:  J Mol Evol       Date:  1986       Impact factor: 2.395

9.  Genome scale reconstruction of a Salmonella metabolic model: comparison of similarity and differences with a commensal Escherichia coli strain.

Authors:  Manal AbuOun; Patrick F Suthers; Gareth I Jones; Ben R Carter; Mark P Saunders; Costas D Maranas; Martin J Woodward; Muna F Anjum
Journal:  J Biol Chem       Date:  2009-08-18       Impact factor: 5.157
  9 in total

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