Literature DB >> 10595558

The effects of alpha-helix on the stability of Asn residues: deamidation rates in peptides of varying helicity.

A A Kosky1, U O Razzaq, M J Treuheit, D N Brems.   

Abstract

Asn deamidation was monitored in Ala-based octadecapeptides of varying alpha-helicity. Gly was substituted for Ala residues at positions 6 and 16 to create a peptide with less helicity. Ala --> Gly substitutions were made at three or more residues from the Asn to negate known primary sequence effects on deamidation rates. The extent of helicity and rate of Asn deamidation for alkaline aqueous solutions of each peptide was measured as a function of temperature by circular dichroism and reversed-phase high-performance liquid chromatography, respectively. The rate of deamidation in the peptides was inversely proportional to the extent of alpha-helicity. The results support the conclusion that Asn deamidation only occurs in the nonhelical population of conformers.

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Year:  1999        PMID： 10595558      PMCID： PMC2144212          DOI： 10.1110/ps.8.11.2519

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  26 in total

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2.  Alpha-helix formation by peptides of defined sequence.

Authors:  R L Baldwin
Journal:  Biophys Chem       Date:  1995 Jun-Jul       Impact factor: 2.352

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Authors:  C L Stevenson; M E Donlan; A R Friedman; R T Borchardt
Journal:  Int J Pept Protein Res       Date:  1993-07

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Journal:  Protein Sci       Date:  1995-11       Impact factor: 6.725

6.  Deuterium exchange of alpha-helices and beta-sheets as monitored by electrospray ionization mass spectrometry.

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Journal:  Protein Sci       Date:  1994-08       Impact factor: 6.725

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Journal:  Pept Res       Date:  1991 Jul-Aug

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Journal:  Protein Sci       Date:  1994-05       Impact factor: 6.725

9.  Deamidation via cyclic imide in asparaginyl peptides.

Authors:  S Capasso; L Mazzarella; F Sica; A Zagari
Journal:  Pept Res       Date:  1989 Mar-Apr

10.  Aromatic side-chain contribution to far-ultraviolet circular dichroism of helical peptides and its effect on measurement of helix propensities.

Authors:  A Chakrabartty; T Kortemme; S Padmanabhan; R L Baldwin
Journal:  Biochemistry       Date:  1993-06-01       Impact factor: 3.162
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  17 in total

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5.  A density functional theory study on peptide bond cleavage at aspartic residues: direct vs cyclic intermediate hydrolysis.

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6.  Multivariate analysis of the sequence dependence of asparagine deamidation rates in peptides.

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7.  Chemical modifications in therapeutic protein aggregates generated under different stress conditions.

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Review 8.  Stability of protein pharmaceuticals: an update.

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9.  Elucidation of degradants in acidic peak of cation exchange chromatography in an IgG1 monoclonal antibody formed on long-term storage in a liquid formulation.

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10.  Deamidation of Human γS-Crystallin Increases Attractive Protein Interactions: Implications for Cataract.

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