Literature DB >> 10595415

Infectious RNA transcripts from grapevine virus A cDNA clone.

N Galiakparov1, E Tanne, I Sela, R Gafny.   

Abstract

A full length cDNA clone of grapevine virus A (GVA) was constructed downstream from the bacteriophage T7 RNA polymerase promoter. Capped in vitro-transcribed RNA was infectious in Nicotiana benthamiana and N. clevelandii plants. Symptoms induced by the RNA transcripts or by the parental virus were indistinguishable. The infectivity of the in vitro-transcribed RNA was confirmed by serological detection of the virus coat and movement proteins and by observation of virions by electron microscopy. This is the first report of infectious RNA transcripts derived from a full-length cDNA clone of a member of the Vitivirus genus.

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Year:  1999        PMID： 10595415     DOI： 10.1023/a:1008192831883

Source DB:  PubMed          Journal:  Virus Genes        ISSN： 0920-8569            Impact factor:   2.332


  9 in total

1.  Vitivirus, a new genus of plant viruses.

Authors:  G P Martelli; A Minafra; P Saldarelli
Journal:  Arch Virol       Date:  1997       Impact factor: 2.574

2.  Replicative form of Semliki Forest virus RNA contains an unpaired guanosine.

Authors:  G Wengler; G Wengler; H S Gross
Journal:  Nature       Date:  1979-12-13       Impact factor: 49.962

Review 3.  Infectious transcripts and cDNA clones of RNA viruses.

Authors:  J C Boyer; A L Haenni
Journal:  Virology       Date:  1994-02       Impact factor: 3.616

4.  A comprehensive set of sequence analysis programs for the VAX.

Authors:  J Devereux; P Haeberli; O Smithies
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

5.  Synthesis of biologically active cDNA clones of cymbidium mosaic potexvirus using a population cloning strategy.

Authors:  H H Yu; S M Wong
Journal:  Arch Virol       Date:  1998       Impact factor: 2.574

6.  Grapevine virus A: nucleotide sequence, genome organization, and relationship in the Trichovirus genus.

Authors:  A Minafra; P Saldarelli; G P Martelli
Journal:  Arch Virol       Date:  1997       Impact factor: 2.574

7.  Nucleotide sequence of the 3' terminal region of the RNA of two filamentous grapevine viruses.

Authors:  A Minafra; P Saldarelli; F Grieco; G P Martelli
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

8.  Serological detection of grapevine virus a using antiserum to a nonstructural protein, the putative movement protein.

Authors:  E Rubinson; N Galiakparov; S Radian; I Sela; E Tanne; R Gafny
Journal:  Phytopathology       Date:  1997-10       Impact factor: 4.025

9.  Double-stranded RNAs of cucumber mosaic virus and its satellite contain an unpaired terminal guanosine: implications for replication.

Authors:  C W Collmer; J M Kaper
Journal:  Virology       Date:  1985-09       Impact factor: 3.616
  9 in total
  3 in total

1.  A universal expression/silencing vector in plants.

Authors:  Yuval Peretz; Rita Mozes-Koch; Fuad Akad; Edna Tanne; Henryk Czosnek; Ilan Sela
Journal:  Plant Physiol       Date:  2007-09-28       Impact factor: 8.340

2.  ORF 5 of grapevine virus A encodes a nucleic acid-binding protein and affects pathogenesis.

Authors:  Nurbol Galiakparov; Edna Tanne; Munir Mawassi; Rony Gafny; Ilan Sela
Journal:  Virus Genes       Date:  2003-12       Impact factor: 2.332

3.  Brief report of the construction of infectious DNA clones of South African genetic variants of grapevine virus A and grapevine virus B.

Authors:  D E Goszczynski
Journal:  Springerplus       Date:  2015-11-26
  3 in total

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