The effects of carbaryl and trichlorphon on differentiating mouse N2a neuroblastoma cells.

The ability of the carbamate pesticide carbaryl (CB) and the organophosphate pesticide trichlorphon (TCL) to inhibit the outgrowth of axon-like processes was studied using mouse N2a neuroblastoma cells induced to differentiate by serum withdrawal. At concentrations of 1 and 2 microg/ml (4.97 and 9.94 microM), CB did not cause cell death but inhibited the outgrowth of axon-like processes from N2a cells. This effect was noted as early as 24 h after exposure of the cells to CB. A similar effect was observed with TCL at concentrations of 1 and 2 microg/ml (3.89 and 7.78 microM). Western blot analysis of cell extracts treated with the pesticides showed decreased cross reactivities with the monoclonal antibody RMd09 compared to control extracts. The results indicate that CB and TCL are both able to inhibit axon development and that this effect is associated with reduced levels of the neurofilament high molecular weight protein subunit (NFH).