| Literature DB >> 10572649 |
M Unger1, E Kartalov, C S Chiu, H A Lester, S R Quake.
Abstract
We demonstrate that it is possible to observe single fluorescent molecules using a standard fluorescence microscope with mercury lamp excitation and an inexpensive cooled charge-coupled device (CCD) camera. With this equipment, we have been able to observe single molecules of tetramethyl-rhodamine, rhodamine 6G, fluorescein isothiocyanate and green fluorescent protein. Immobilized molecules were observed both in air and in aqueous solution.Entities:
Mesh：
Substances：
Year: 1999 PMID： 10572649 DOI： 10.2144/99275rr01
Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993