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Literature DB >> 10557291

DNA transport by a micromachined Brownian ratchet device.

J S Bader¹, R W Hammond, S A Henck, M W Deem, G A McDermott, J M Bustillo, J W Simpson, G T Mulhern, J M Rothberg.

Abstract

We have micromachined a silicon-chip device that transports DNA with a Brownian ratchet that rectifies the Brownian motion of microscopic particles. Transport properties for a DNA 50-mer agree with theoretical predictions, and the DNA diffusion constant agrees with previous experiments. This type of micromachine could provide a generic pump or separation component for DNA or other charged species as part of a microscale lab-on-a-chip. A device with reduced feature size could produce a size-based separation of DNA molecules, with applications including the detection of single-nucleotide polymorphisms.

Entities: Chemical

Mesh：

Substances：
DNA

Year: 1999 PMID： 10557291 PMCID： PMC23918 DOI： 10.1073/pnas.96.23.13165

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

DNA transport by a micromachined Brownian ratchet device.

1. Precise numerics versus theory for correlation ratchets.

2. Dielectrophoretic ratchets.

3. Fluctuation driven ratchets: Molecular motors.

4. Variations on a theme: cataloging human DNA sequence variation.

5. The future of genetic studies of complex human diseases.

6. Movement of microtubules by single kinesin molecules.

7. Direct observation of kinesin stepping by optical trapping interferometry.

8. Directional motion of brownian particles induced by a periodic asymmetric potential.

9. Brownian ratchets: molecular separations in lipid bilayers supported on patterned arrays.

10. Mechanochemical coupling of the motion of molecular motors to ATP hydrolysis.

1. A coarse-grained model for a nanometer-scale molecular pump.

2. Organic electronic ratchets doing work.

3. Translocation of a granular chain in a horizontally vibrated saw-tooth channel.

Review 4. Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.

5. Ratchet rectification effect on the translocation of a flexible polyelectrolyte chain.

6. Light-responsive organic flashing electron ratchet.

7. Regulating Brownian fluctuations with tunable microscopic magnetic traps.

Review 8. Brownian ratchet mechanisms of ParA-mediated partitioning.

9. Diffusion of DNA molecules in gel at high electric fields.

10. Polymer properties of polythymine as revealed by translational diffusion.