Literature DB >> 10544034

Construction of Epstein-Barr virus-based expression vector containing mini-oriP.

J Tanaka1, Y Miwa, K Miyoshi, A Ueno, H Inoue.   

Abstract

Epstein-Barr virus (EBV)-based vectors are extrachromosomal vectors carrying a replicational origin, oriP (about 2200 bp) and a replication initiation factor (EBNA-1) which are sufficient for autonomous replication. Because one disadvantage of these vectors is their large sizes, we examined the effect of partial deletion of oriP on the effectiveness of the EBV-based vectors, using an enhanced green fluorescent protein (EGFP) as a reporter to monitor gene expression. Results indicated that 954 bp-deleted mini-oriP is useful in primate cells since the vector showed high efficiency of stable transfection, a high ratio of EGFP-positive cells, and high recovery of intact plasmid DNA from transfected cells. Copyright 1999 Academic Press.

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Year:  1999        PMID： 10544034     DOI： 10.1006/bbrc.1999.1617

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  17 in total

1.  Two distinct action mechanisms of immunophilin-ligand complexes for the blockade of T-cell activation.

Authors:  S Matsuda; F Shibasaki; K Takehana; H Mori; E Nishida; S Koyasu
Journal:  EMBO Rep       Date:  2000-11       Impact factor: 8.807

2.  Easy stable transfection of a human cancer cell line by electrogene transfer with an Epstein-Barr virus-based plasmid vector.

Authors:  Masa-Aki Shibata; Yoshihiro Miwa; Junji Morimoto; Yoshinori Otsuki
Journal:  Med Mol Morphol       Date:  2007-06-18       Impact factor: 2.309

3.  Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase.

Authors:  Hidekazu Tsutsui; Yuka Jinno; Akiko Tomita; Yusuke Niino; Yoshiyuki Yamada; Katsuhiko Mikoshiba; Atsushi Miyawaki; Yasushi Okamura
Journal:  J Physiol       Date:  2013-07-08       Impact factor: 5.182

4.  Dual gene expression in embryoid bodies derived from human induced pluripotent stem cells using episomal vectors.

Authors:  Minoru Tomizawa; Fuminobu Shinozaki; Yasufumi Motoyoshi; Takao Sugiyama; Shigenori Yamamoto; Makoto Sueishi
Journal:  Tissue Eng Part A       Date:  2014-12       Impact factor: 3.845

5.  CD26 mediates dissociation of Tollip and IRAK-1 from caveolin-1 and induces upregulation of CD86 on antigen-presenting cells.

Authors:  Kei Ohnuma; Tadanori Yamochi; Masahiko Uchiyama; Kunika Nishibashi; Satoshi Iwata; Osamu Hosono; Hiroshi Kawasaki; Hirotoshi Tanaka; Nam H Dang; Chikao Morimoto
Journal:  Mol Cell Biol       Date:  2005-09       Impact factor: 4.272

6.  CD26 up-regulates expression of CD86 on antigen-presenting cells by means of caveolin-1.

Authors:  Kei Ohnuma; Tadanori Yamochi; Masahiko Uchiyama; Kunika Nishibashi; Noritada Yoshikawa; Noriaki Shimizu; Satoshi Iwata; Hirotoshi Tanaka; Nam H Dang; Chikao Morimoto
Journal:  Proc Natl Acad Sci U S A       Date:  2004-09-07       Impact factor: 11.205

7.  Negative feedback loop in T-cell activation through MAPK-catalyzed threonine phosphorylation of LAT.

Authors:  Satoshi Matsuda; Yoshihiro Miwa; Yasuko Hirata; Akiko Minowa; Junko Tanaka; Eisuke Nishida; Shigeo Koyasu
Journal:  EMBO J       Date:  2004-06-10       Impact factor: 11.598

8.  Amplification of EBNA-1 through a single-plasmid vector-based gene amplification system in HEK293 cells as an efficient transient gene expression system.

Authors:  Sun-Hye Park; Jong-Ho Park; Joo-Hyoung Lee; Hoon-Min Lee; Yeon-Ju Kang; Eun-Ji Lee; Seunghyeon Shin; Gyun Min Lee; Yeon-Gu Kim
Journal:  Appl Microbiol Biotechnol       Date:  2020-11-16       Impact factor: 4.813

9.  An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease.

Authors:  Reiko Onuki; Yoshio Bando; Eigo Suyama; Taiichi Katayama; Hiroaki Kawasaki; Tadashi Baba; Masaya Tohyama; Kazunari Taira
Journal:  EMBO J       Date:  2004-02-05       Impact factor: 11.598

10.  Identification of rTid-1, the rat homologue of the drosophila tumor suppressor l(2)tid gene.

Authors:  Masako Fujita; Yasuo Nagai; Tohru Sawada; Klaus Heese
Journal:  Mol Cell Biochem       Date:  2004-03       Impact factor: 3.396
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