Literature DB >> 10530800

Preparation of highly purified momordin II without ribonuclease activity.

P Valbonesi1, L Barbieri, A Bolognesi, E Bonora, L Polito, F Stirpe.   

Abstract

Momordin II, a ribosome-inactivating protein from Momordica charantia seeds, was purified by a procedure involving a series of chromatographies on S-Sepharose, Sephadex G-50, CM-Sepharose, and Red Sepharose columns. Highly purified momordin II inhibited cell-free protein synthesis, released adenine from rat liver ribosomes and from DNA, and had no RNase activity.

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Year:  1999        PMID: 10530800     DOI: 10.1016/s0024-3205(99)00389-6

Source DB:  PubMed          Journal:  Life Sci        ISSN: 0024-3205            Impact factor:   5.037


  5 in total

Review 1.  Ribosome-inactivating proteins in plant biology.

Authors:  Sang-Wook Park; Ramarao Vepachedu; Neelam Sharma; Jorge M Vivanco
Journal:  Planta       Date:  2004-09-03       Impact factor: 4.116

2.  Biological activities of the antiviral protein BE27 from sugar beet (Beta vulgaris L.).

Authors:  Rosario Iglesias; Lucía Citores; Antimo Di Maro; José M Ferreras
Journal:  Planta       Date:  2014-10-19       Impact factor: 4.116

3.  Soapwort Saporin L3 Expression in Yeast, Mutagenesis, and RNA Substrate Specificity.

Authors:  Hongling Yuan; Quan Du; Matthew B Sturm; Vern L Schramm
Journal:  Biochemistry       Date:  2015-07-14       Impact factor: 3.162

Review 4.  Ribosome-inactivating and related proteins.

Authors:  Joachim Schrot; Alexander Weng; Matthias F Melzig
Journal:  Toxins (Basel)       Date:  2015-05-08       Impact factor: 4.546

Review 5.  Plant Ribosome-Inactivating Proteins: Progesses, Challenges and Biotechnological Applications (and a Few Digressions).

Authors:  Maria Serena Fabbrini; Miku Katayama; Ikuhiko Nakase; Riccardo Vago
Journal:  Toxins (Basel)       Date:  2017-10-12       Impact factor: 4.546

  5 in total

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