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Literature DB >> 10516075

Association of murine leukemia virus pol with virions, independent of Gag-Pol expression.

G L Buchschacher¹, L Yu, F Murai, T Friedmann, A Miyanohara.

Abstract

During the replication cycle of murine leukemia virus (MLV), Pol is normally synthesized as part of a Gag-Pol fusion protein. In this study, the ability of free MLV Pol to be incorporated into virions was examined. When MLV Gag and MLV Pol were coexpressed from separate plasmids in cells, reverse transcriptase (RT) activity associated with Gag core particles at a slightly lower level than did RT activity generated from wild-type Gag-Pol expression. Particles produced in this manner were somewhat less infectious than those produced with wild-type Gag-Pol. A smaller amount of MLV Pol also associated with heterologous human immunodeficiency virus type 1 Gag cores.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1999 PMID： 10516075 PMCID： PMC113001

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

34 in total

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Authors: A Bukovsky; H Göttlinger
Journal: J Virol Date: 1996-10 Impact factor: 5.103

Review 2. Proteolytic processing and particle maturation.

Authors: V M Vogt
Journal: Curr Top Microbiol Immunol Date: 1996 Impact factor: 4.291

3. Efficient infection of a human T-cell line and of human primary peripheral blood leukocytes with a pseudotyped retrovirus vector.

Authors: S Sharma; M Cantwell; T J Kipps; T Friedmann
Journal: Proc Natl Acad Sci U S A Date: 1996-10-15 Impact factor: 11.205

4. Functional RT and IN incorporated into HIV-1 particles independently of the Gag/Pol precursor protein.

Authors: X Wu; H Liu; H Xiao; J A Conway; E Hunter; J C Kappes
Journal: EMBO J Date: 1997-08-15 Impact factor: 11.598

5. Inactivation of the human immunodeficiency virus type 1 inhibitory elements allows Rev-independent expression of Gag and Gag/protease and particle formation.

Authors: R Schneider; M Campbell; G Nasioulas; B K Felber; G N Pavlakis
Journal: J Virol Date: 1997-07 Impact factor: 5.103

6. Complementation of integrase function in HIV-1 virions.

Authors: T M Fletcher; M A Soares; S McPhearson; H Hui; M Wiskerchen; M A Muesing; G M Shaw; A D Leavitt; J D Boeke; B H Hahn
Journal: EMBO J Date: 1997-08-15 Impact factor: 11.598

7. Incorporation of Pr160(gag-pol) into virus particles requires the presence of both the major homology region and adjacent C-terminal capsid sequences within the Gag-Pol polyprotein.

Authors: M Huang; M A Martin
Journal: J Virol Date: 1997-06 Impact factor: 5.103

8. Noninfectious virus-like particles produced by Moloney murine leukemia virus-based retrovirus packaging cells deficient in viral envelope become infectious in the presence of lipofection reagents.

Authors: S Sharma; F Murai; A Miyanohara; T Friedmann
Journal: Proc Natl Acad Sci U S A Date: 1997-09-30 Impact factor: 11.205

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Authors: A S Reicin; A Ohagen; L Yin; S Hoglund; S P Goff
Journal: J Virol Date: 1996-12 Impact factor: 5.103

10. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector.

Authors: L Naldini; U Blömer; P Gallay; D Ory; R Mulligan; F H Gage; I M Verma; D Trono
Journal: Science Date: 1996-04-12 Impact factor: 47.728

10 in total

1. Transduction efficiency of pantropic retroviral vectors is controlled by the envelope plasmid to vector plasmid ratio.

Authors: Yong Chen; William M Miller; Ashok Aiyar
Journal: Biotechnol Prog Date: 2005 Jan-Feb

2. Gag-Pol supplied in trans is efficiently packaged and supports viral function in human immunodeficiency virus type 1.

Authors: M K Hill; C W Hooker; D Harrich; S M Crowe; J Mak
Journal: J Virol Date: 2001-08 Impact factor: 5.103

3. Coding sequences upstream of the human immunodeficiency virus type 1 reverse transcriptase domain in Gag-Pol are not essential for incorporation of the Pr160(gag-pol) into virus particles.

Authors: Hsu-Chen Chiu; Szu-Yung Yao; Chin-Tien Wang
Journal: J Virol Date: 2002-04 Impact factor: 5.103

4. Murine leukemia virus particle assembly quantitated by fluorescence microscopy: role of Gag-Gag interactions and membrane association.

Authors: Mariam Andrawiss; Yasuhiro Takeuchi; Lindsay Hewlett; Mary Collins
Journal: J Virol Date: 2003-11 Impact factor: 5.103

5. Pregenomic RNA is required for efficient incorporation of pol polyprotein into foamy virus capsids.

Authors: Martin Heinkelein; Cordula Leurs; Matthias Rammling; Katrin Peters; Helmut Hanenberg; Axel Rethwilm
Journal: J Virol Date: 2002-10 Impact factor: 5.103

6. A single amino acid substitution in HIV-1 reverse transcriptase significantly reduces virion release.

Authors: Chien-Cheng Chiang; Shiu-Mei Wang; Yen-Yu Pan; Kuo-Jung Huang; Chin-Tien Wang
Journal: J Virol Date: 2009-11-04 Impact factor: 5.103

7. Incorporation of pol into human immunodeficiency virus type 1 Gag virus-like particles occurs independently of the upstream Gag domain in Gag-pol.

Authors: Shan Cen; Meijuan Niu; Jenan Saadatmand; Fei Guo; Yue Huang; Gary J Nabel; Lawrence Kleiman
Journal: J Virol Date: 2004-01 Impact factor: 5.103