Literature DB >> 10508107

Dephosphorylation of phytate by using the Aspergillus niger phytase with a high affinity for phytate.

T Nagashima1, T Tange, H Anazawa.   

Abstract

A phytase (EC 3.1.3.8) with a high affinity for phytic acid was found in Aspergillus niger SK-57 and purified to homogeneity in four steps by using ion-exchange chromatography (two types), gel filtration, and chromatofocusing. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme gave a single stained band at a molecular mass of approximately 60 kDa. The Michaelis constant of the enzyme for phytic acid (18.7 +/- 4.6 microM) was statistically analyzed. In regard to the orthophosphate released from phytic acid, a significant difference between a low K(m) phytase from A. niger SK-57 and a high K(m) phytase from Aspergillus ficuum was recognized.

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Year:  1999        PMID： 10508107      PMCID： PMC91625          DOI： 10.1128/AEM.65.10.4682-4684.1999

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

1.  Crystal structure of phytase from Aspergillus ficuum at 2.5 A resolution.

Authors:  D Kostrewa; F Grüninger-Leitch; A D'Arcy; C Broger; D Mitchell; A P van Loon
Journal:  Nat Struct Biol       Date:  1997-03

2.  Extracellular phytase (E.C. 3.1.3.8) from Aspergillus ficuum NRRL 3135: purification and characterization.

Authors:  A H Ullah; D M Gibson
Journal:  Prep Biochem       Date:  1987

3.  Inositol phosphate phosphatases of microbiological origin. Some properties of a partially purified bacterial (Pseudomonas sp.) phytase.

Authors:  G C Irving; D J Cosgrove
Journal:  Aust J Biol Sci       Date:  1971-06

4.  The phytase subfamily of histidine acid phosphatases: isolation of genes for two novel phytases from the fungi Aspergillus terreus and Myceliophthora thermophila.

Authors:  David B Mitchell; Kurt Vogel; Bernd J Weimann; Luis Pasamontes; Adolphus P G M van Loon
Journal:  Microbiology (Reading)       Date:  1997-01       Impact factor: 2.777

5.  The cloning and sequencing of the genes encoding phytase (phy) and pH 2.5-optimum acid phosphatase (aph) from Aspergillus niger var. awamori.

Authors:  C S Piddington; C S Houston; M Paloheimo; M Cantrell; A Miettinen-Oinonen; H Nevalainen; J Rambosek
Journal:  Gene       Date:  1993-10-29       Impact factor: 3.688

6.  Purification and properties of phytate-specific phosphatase from Bacillus subtilis.

Authors:  V K Powar; V Jagannathan
Journal:  J Bacteriol       Date:  1982-09       Impact factor: 3.490

7.  Cloning, characterization and overexpression of the phytase-encoding gene (phyA) of Aspergillus niger.

Authors:  W van Hartingsveldt; C M van Zeijl; G M Harteveld; R J Gouka; M E Suykerbuyk; R G Luiten; P A van Paridon; G C Selten; A E Veenstra; R F van Gorcom
Journal:  Gene       Date:  1993-05-15       Impact factor: 3.688

8.  Purification and characterization of two phytases from Escherichia coli.

Authors:  R Greiner; U Konietzny; K D Jany
Journal:  Arch Biochem Biophys       Date:  1993-05-15       Impact factor: 4.013

9.  Aspergillus ficuum phytase: partial primary structure, substrate selectivity, and kinetic characterization.

Authors:  A H Ullah
Journal:  Prep Biochem       Date:  1988
  9 in total
  6 in total

1.  Biochemical characterisation of extracellular phytase (myo-inositol hexakisphosphate phosphohydrolase) from a hyper-producing strain of Aspergillus niger van Teighem.

Authors:  Purva Vats; U C Banerjee
Journal:  J Ind Microbiol Biotechnol       Date:  2005-03-18       Impact factor: 3.346

2.  Extracellular phytase from Aspergillus niger CFR 335: purification and characterization.

Authors:  B S Gunashree; G Venkateswaran
Journal:  J Food Sci Technol       Date:  2014-08-06       Impact factor: 2.701

3.  Overexpression and functional characterization of an Aspergillus niger phytase in the fat body of transgenic silkworm, Bombyx mori.

Authors:  Hanfu Xu; Yaowen Liu; Feng Wang; Lin Yuan; Yuancheng Wang; Sanyuan Ma; Helen Beneš; QingYou Xia
Journal:  Transgenic Res       Date:  2014-04-10       Impact factor: 2.788

4.  Heat processing increased the digestibility of phosphorus in soybean expeller, canola meal, and canola expeller fed to growing pigs.

Authors:  Jinyoung Lee; Charles Martin Nyachoti
Journal:  J Anim Sci       Date:  2021-10-01       Impact factor: 3.338

Review 5.  Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A Review.

Authors:  Gregor Marolt; Mitja Kolar
Journal:  Molecules       Date:  2020-12-31       Impact factor: 4.411

6.  The Fish Pathogen Aliivibrio salmonicida LFI1238 Can Degrade and Metabolize Chitin despite Gene Disruption in the Chitinolytic Pathway.

Authors:  Anna Skåne; Giusi Minniti; Jennifer S M Loose; Sophanit Mekasha; Bastien Bissaro; Geir Mathiesen; Magnus Ø Arntzen; Gustav Vaaje-Kolstad
Journal:  Appl Environ Microbiol       Date:  2021-09-10       Impact factor: 4.792
  6 in total

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