Literature DB >> 10508043

Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis.

P Scarpellini1, S Braglia, P Carrera, M Cedri, P Cichero, A Colombo, R Crucianelli, A Gori, M Ferrari, A Lazzarin.   

Abstract

We applied double gradient-denaturing gradient gel electrophoresis (DG-DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.

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Year:  1999        PMID： 10508043      PMCID： PMC89519          DOI： 10.1128/AAC.43.10.2550

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  22 in total

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Authors:  J M Hunt; G D Roberts; L Stockman; T A Felmlee; D H Persing
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Authors:  V Kapur; L L Li; S Iordanescu; M R Hamrick; A Wanger; B N Kreiswirth; J M Musser
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8.  DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.

Authors:  S G Fischer; L S Lerman
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Authors:  D J Jin; C A Gross
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10.  Direct, automated detection of rifampin-resistant Mycobacterium tuberculosis by polymerase chain reaction and single-strand conformation polymorphism analysis.

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6.  Detection of rpoB mutations associated with rifampin resistance in Mycobacterium tuberculosis using denaturing gradient gel electrophoresis.

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10.  Rapid detection of rpoB mutations in rifampin resistant M. tuberculosis from sputum samples by denaturing gradient gel electrophoresis.

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