Literature DB >> 10503242

Isolation and analysis of amplified cDNA fragments during detection of unknown polymorphisms with temperature modulated heteroduplex chromatography.

A Kuklin1, K Munson, P Taylor, D Gjerde.   

Abstract

A procedure for isolation of polymerase chain reaction (PCR) fragments from an automated system for DNA fragment analysis is described. The technique is used for screening of unknown mutations or polymorphisms. Fragments are isolated during the analysis from the system by an automated unit for high-throughput projects or manually when working with a few samples.

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Year:  1999        PMID： 10503242     DOI： 10.1007/BF02788684

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  5 in total

Review 1.  DNA variation and the future of human genetics.

Authors:  A J Schafer; J R Hawkins
Journal:  Nat Biotechnol       Date:  1998-01       Impact factor: 54.908

2.  Large-scale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome.

Authors:  D G Wang; J B Fan; C J Siao; A Berno; P Young; R Sapolsky; G Ghandour; N Perkins; E Winchester; J Spencer; L Kruglyak; L Stein; L Hsie; T Topaloglou; E Hubbell; E Robinson; M Mittmann; M S Morris; N Shen; D Kilburn; J Rioux; C Nusbaum; S Rozen; T J Hudson; R Lipshutz; M Chee; E S Lander
Journal:  Science       Date:  1998-05-15       Impact factor: 47.728

Review 3.  Slowly but surely towards better scanning for mutations.

Authors:  R G Cotton
Journal:  Trends Genet       Date:  1997-02       Impact factor: 11.639

4.  Sequencing using capillary electrophoresis of short tandem repeat alleles separated and purified by high performance liquid chromatography.

Authors:  M A Marino; J M Devaney; J K Smith; J E Girard
Journal:  Electrophoresis       Date:  1998-01       Impact factor: 3.535
  5 in total

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