Literature DB >> 10499441

Evidence of focal genetic microheterogeneity in glioblastoma multiforme by area-specific CGH on microdissected tumor cells.

V Jung1, B F Romeike, W Henn, W Feiden, J R Moringlane, K D Zang, S Urbschat.   

Abstract

The term "multiforme" in glioblastoma multiforme (GBM) indicates the highly variable histomorphology that cannot be addressed by studies on homogenized tissue probes. In order to relate genetic findings with histomorphologically distinct areas we used microdissection to procure defined cell populations from microscopic tissue sections under direct visualization. Formalin-fixed and paraffin-embedded tissue sections of 10 GBM were evaluated for intratumoral genetic heterogeneity by microdissection of multiple areas of 20-50 tumor cells and DOP-PCR of DNA isolated from the dissected cell groups, followed by comparative genomic hybridization (CGH). Microdissected cells from histomorphologically normal extratumoral blood vessels from the same slides served as controls. The individual tumors showed variable combinations of primary chromosomal gains and losses common to all studied areas of a given case along with secondary, area-specific additional aberrations. CGH displayed a wider variety of chromosomal aberrations than metaphase cytogenetics of cell cultures from the same tumors. The most frequent aberrations observed were previously unperceived gains on chromosomes 4q (8/10) and 5q (5/10). Other nonrandom aberrations were gains on 12q (6/10), 13q (6/10), and 7 (5/10), and losses of 22 (5/10). Amplifications on 7p were intratumorally heterogeneous and only found in single areas of 2 tumors. In contrast to normal extratumoral vessels, vascular proliferates in most cases demonstrated chromosomal aberrations (CGH) which were partially different from the aberrations observed in the tumor itself. The described method gives evidence of considerable intratumoral genetic heterogeneity in GBM and provides a sensitive tool for the detection of quantitative chromosomal changes that are present only regionally within a given tumor.

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Year:  1999        PMID: 10499441     DOI: 10.1097/00005072-199909000-00009

Source DB:  PubMed          Journal:  J Neuropathol Exp Neurol        ISSN: 0022-3069            Impact factor:   3.685


  34 in total

1.  Identification of uncommon chromosomal aberrations in the neuroglioma cell line H4 by spectral karyotyping.

Authors:  D Krex; B Mohr; M Hauses; G Ehninger; H K Schackert; G Schackert
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2.  Extensive intra-tumor heterogeneity in primary human glial tumors as a result of locus non-specific genomic alterations.

Authors:  A Misra; P Chattopadhyay; A K Dinda; C Sarkar; A K Mahapatra; S E Hasnain; S Sinha
Journal:  J Neurooncol       Date:  2000-05       Impact factor: 4.130

3.  Support vector machine multiparametric MRI identification of pseudoprogression from tumor recurrence in patients with resected glioblastoma.

Authors:  Xintao Hu; Kelvin K Wong; Geoffrey S Young; Lei Guo; Stephen T Wong
Journal:  J Magn Reson Imaging       Date:  2011-02       Impact factor: 4.813

Review 4.  Heterogeneity maintenance in glioblastoma: a social network.

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Journal:  Cancer Res       Date:  2011-05-31       Impact factor: 12.701

Review 5.  Insights into molecular therapy of glioma: current challenges and next generation blueprint.

Authors:  Y Rajesh; Ipsita Pal; Payel Banik; Sandipan Chakraborty; Sachin A Borkar; Goutam Dey; Ahona Mukherjee; Mahitosh Mandal
Journal:  Acta Pharmacol Sin       Date:  2017-03-20       Impact factor: 6.150

6.  F-18 FDG PET-CT for predicting survival in patients with recurrent glioma: a prospective study.

Authors:  Amburanjan Santra; Rakesh Kumar; Punit Sharma; Chandrashekhar Bal; Pramod Kumar Julka; Arun Malhotra
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7.  Pediatric high-grade astrocytomas show chromosomal imbalances distinct from adult cases.

Authors:  C H Rickert; R Sträter; P Kaatsch; H Wassmann; H Jürgens; B Dockhorn-Dworniczak; W Paulus
Journal:  Am J Pathol       Date:  2001-04       Impact factor: 4.307

8.  Glioblastoma heterogeneity and more accurate representation in research models.

Authors:  Hugo Guerrero-Cázares; Linda Chen; Alfredo Quiñones-Hinojosa
Journal:  World Neurosurg       Date:  2011-11-01       Impact factor: 2.104

9.  The importance of genomic copy number changes in the prognosis of glioblastoma multiforme.

Authors:  Ali Arslantas; Sevilhan Artan; Ulkü Oner; Hamza Müslümanoğlu; Ramazan Durmaz; Erhan Cosan; Metin Ant Atasoy; Nurettin Başaran; Eşref Tel
Journal:  Neurosurg Rev       Date:  2003-07-04       Impact factor: 3.042

10.  Subtelomeric analysis of pediatric astrocytoma: subchromosomal instability is a distinctive feature of pleomorphic xanthoastrocytoma.

Authors:  Elena Grau; J Balaguer; A Canete; F Martinez; C Orellana; S Oltra; M Hernandez; V Castel
Journal:  J Neurooncol       Date:  2008-12-20       Impact factor: 4.130

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