Literature DB >> 10499094

Expression of 15N-labeled eukaryotic cytochrome c in Escherichia coli.

A S Morar1, D Kakouras, G B Young, J Boyd, G J Pielak.   

Abstract

We present a simple and inexpensive method for producing 15N-labeled Saccharomyces cerevisiae iso-1-cytochrome c in Escherichia coli. The labeled protein gives excellent NMR spectra.

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Year:  1999        PMID： 10499094     DOI： 10.1007/s007750050307

Source DB:  PubMed          Journal:  J Biol Inorg Chem        ISSN： 0949-8257            Impact factor:   3.358


  17 in total

1.  Second virial coefficients as a measure of protein--osmolyte interactions.

Authors:  G T Weatherly; G J Pielak
Journal:  Protein Sci       Date:  2001-01       Impact factor: 6.725

2.  A further investigation of the cytochrome b5-cytochrome c complex.

Authors:  Lucia Banci; Ivano Bertini; Isabella C Felli; Ludwig Krippahl; Karel Kubicek; José J G Moura; Antonio Rosato
Journal:  J Biol Inorg Chem       Date:  2003-07-19       Impact factor: 3.358

3.  Backbone and side-chain heteronuclear resonance assignments and hyperfine NMR shifts in horse cytochrome c.

Authors:  Weixia Liu; Jon Rumbley; S Walter Englander; A Joshua Wand
Journal:  Protein Sci       Date:  2003-09       Impact factor: 6.725

4.  Redox-dependent conformational changes in eukaryotic cytochromes revealed by paramagnetic NMR spectroscopy.

Authors:  Alexander N Volkov; Sophie Vanwetswinkel; Karen Van de Water; Nico A J van Nuland
Journal:  J Biomol NMR       Date:  2012-02-10       Impact factor: 2.835

5.  Solution structure and dynamics of the complex between cytochrome c and cytochrome c peroxidase determined by paramagnetic NMR.

Authors:  Alexander N Volkov; Jonathan A R Worrall; Elodie Holtzmann; Marcellus Ubbink
Journal:  Proc Natl Acad Sci U S A       Date:  2006-12-04       Impact factor: 11.205

6.  Effect of alternative distal residues on the reactivity of cytochrome c peroxidase: properties of CcP mutants H52D, H52E, H52N, and H52Q.

Authors:  Miriam C Foshay; Lidia B Vitello; James E Erman
Journal:  Biochim Biophys Acta       Date:  2011-02-24

7.  Expression and characterization of recombinant human cytochrome c in E. coli.

Authors:  Wen-Yih Jeng; Chiu-Yueh Chen; Hsien-Chang Chang; Woei-Jer Chuang
Journal:  J Bioenerg Biomembr       Date:  2002-12       Impact factor: 2.945

8.  Apolar distal pocket mutants of yeast cytochrome c peroxidase: hydrogen peroxide reactivity and cyanide binding of the TriAla, TriVal, and TriLeu variants.

Authors:  Anil K Bidwai; Cassandra Meyen; Heather Kilheeney; Damian Wroblewski; Lidia B Vitello; James E Erman
Journal:  Biochim Biophys Acta       Date:  2012-09-25

9.  The orientations of cytochrome c in the highly dynamic complex with cytochrome b5 visualized by NMR and docking using HADDOCK.

Authors:  Alexander N Volkov; Davide Ferrari; Jonathan A R Worrall; Alexandre M J J Bonvin; Marcellus Ubbink
Journal:  Protein Sci       Date:  2005-02-02       Impact factor: 6.725

10.  The determinants of stability and folding in evolutionarily diverged cytochromes c.

Authors:  Megan C Thielges; Jörg Zimmermann; Philip E Dawson; Floyd E Romesberg
Journal:  J Mol Biol       Date:  2009-03-04       Impact factor: 5.469
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