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Literature DB >> 10493116

Detection of Taenia solium cysticercosis in pigs by ELISA with an excretory-secretory antigen.

Abstract

Serodiagnosis of Taenia solium cysticercosis in pigs was conducted by an enzyme-linked immunosorbent assay with an excretory-secretory antigen. The antigen was obtained by in vitro cultivation of the cysticerci in a synthetic medium RPMI 1640. The sensitivity and specificity of the ELISA in detecting infection in pigs reared on free range was 92% and 100%, respectively. In addition, 33.33% of pigs in which infection could not be detected at meat inspection were found positive by ELISA. However, none of the sera from a group of farm-reared pigs were positive. No cross reactions were observed in pigs that contained either the cysticerci of Taenia hydatigena or hydatid cysts.

Entities: Chemical Disease Species

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Year: 1999 PMID： 10493116 DOI： 10.1023/a:1006366920111

Source DB: PubMed Journal: Vet Res Commun ISSN： 0165-7380 Impact factor: 2.459

7 in total

1. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

2. Presentation of a membrane cysticercus antigen and its homology with excretory--secretory antigen.

Authors: N A Khan; J Sotelo
Journal: Acta Leiden Date: 1989

3. Serodiagnosis of cysticercosis: specificity of different antigens and enzyme-linked immunosorbent assays.

Authors: T F Ng; R C Ko
Journal: Trans R Soc Trop Med Hyg Date: 1994 Jul-Aug Impact factor: 2.184

4. Antibody levels in cattle naturally infected with Taenia saginata metacestodes in Britain.

Authors: L J Harrison; M M Sewell
Journal: Res Vet Sci Date: 1981-07 Impact factor: 2.534

5. Diagnosis of swine trichinosis by enzyme-linked immunosorbent assay (ELISA) using an excretory--secretory antigen.

Authors: H R Gamble; W R Anderson; C E Graham; K D Murrell
Journal: Vet Parasitol Date: 1983-11 Impact factor: 2.738

Review 6. Excretory-secretory products of helminth parasites: effects on host immune responses.

Authors: M W Lightowlers; M D Rickard
Journal: Parasitology Date: 1988 Impact factor: 3.234

7. The use of excretory and secretory antigens of the scolex of Taenia ovis for the serodiagnosis of infection in dogs.

Authors: D D Heath; S B Lawrence; A Glennie; H Twaalfhoven
Journal: J Parasitol Date: 1985-04 Impact factor: 1.276

7 in total

5 in total

Detection of Taenia solium cysticercosis in pigs by ELISA with an excretory-secretory antigen.

1. Protein measurement with the Folin phenol reagent.

2. Presentation of a membrane cysticercus antigen and its homology with excretory--secretory antigen.

3. Serodiagnosis of cysticercosis: specificity of different antigens and enzyme-linked immunosorbent assays.

4. Antibody levels in cattle naturally infected with Taenia saginata metacestodes in Britain.

5. Diagnosis of swine trichinosis by enzyme-linked immunosorbent assay (ELISA) using an excretory--secretory antigen.

Review 6. Excretory-secretory products of helminth parasites: effects on host immune responses.

7. The use of excretory and secretory antigens of the scolex of Taenia ovis for the serodiagnosis of infection in dogs.

1. Cloning and characterization of Clonorchis sinensis myoglobin using immune sera against excretory-secretory antigens.

2. Relationship between serum antibodies and Taenia solium larvae burden in pigs raised in field conditions.

3. Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis.

4. Pediatric neurocysticercosis: usefulness of antibody response in cysticidal treatment follow-up.

Review 5. Monitoring the outcomes of interventions against Taenia solium: options and suggestions.