| Literature DB >> 10470338 |
T Jelinek1, A H Kilian, A Westermeier, S Pröll, G Kabagambe, H D Nothdurft, F von Sonnenburg, T Löscher.
Abstract
It has been proposed that polymorphisms of the Merozoite Surface Protein 1 and 2 (MSP1 and MSP2) and the Glutamate Rich Protein (GLURP) genes can be considered as genetic markers for the genotyping of field populations of Plasmodium falciparum. During a field study on in vivo drug resistance against chloroquine, sulphadoxine/pyrimethamine (S/P) and cotrimoxazole in West Uganda, sensitive and resistant isolates were collected from patients by fingerprick for genotyping. 59 (72.8%) of the 81 P. falciparum samples isolated at day 0 showed multiclonal infection with 2-7 clones. Among the isolates we investigated, presence of the allelic family MAD20 of MSP1 at day 0 was significantly (P = 0.0041) associated with decreased resistance to antimalarials. Use of this method in a field study on in vivo drug resistance demonstrates another potential application of genotyping as a tool for epidemiological investigations.Entities:
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Year: 1999 PMID： 10470338 DOI： 10.1046/j.1365-3156.1999.00428.x
Source DB: PubMed Journal: Trop Med Int Health ISSN： 1360-2276 Impact factor: 2.622