| Literature DB >> 10450438 |
A T Jensen1, S Gasim, T Moller, A Ismail, A Gaafar, M Kemp, A M el Hassan, A Kharazmi, T M Alce, D F Smith, T G Theander.
Abstract
The repetitive sequence of Leishmania major gene B protein (GBP) has previously been shown to be a useful tool in the diagnosis of cutaneous leishmaniasis (CL). Here, we have assessed enzyme-linked immunosorbent assays (ELISAs) using recombinant L. donovani GBP (rGBP) and a peptide sequence of L. donovani GBP (GBPP) in the diagnosis of L. donovani infections in Sudan. The sensitivity of the rGBP ELISA in diagnosing visceral leishmaniasis (VL) and post kala-azar dermal leishmaniasis (PKDL) was 92% and 93%, respectively. In contrast, the sensitivity of the GBPP ELISA was 55% for VL and 63% for PKDL. Plasma antibody reactivity of donors with VL and PKDL remained high for an extended period after the end of treatment. Antibody-reactivity to rGBP and GBPP was detected in 71% and 14% of plasma samples from CL patients, respectively. Plasma from healthy Sudanese donors living in an area endemic for malaria but free of leishmaniasis was negative in both assays.Entities:
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Year: 1999 PMID： 10450438 DOI： 10.1016/s0035-9203(99)90291-2
Source DB: PubMed Journal: Trans R Soc Trop Med Hyg ISSN： 0035-9203 Impact factor: 2.184