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Literature DB >> 10439510

Matrix-assisted laser desorption ionization hydrogen/deuterium exchange studies to probe peptide conformational changes.

Abstract

Hydrogen/deuterium (H/D) exchange chemistry monitored by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry is used to study solution phase conformational changes of bradykinin, alpha-melanocyte stimulating hormone, and melittin as water is added to methanol-d4, acetonitrile, and isopropanol-d8 solutions. The results are interpreted in terms of a preference for the peptides to acquire more compact conformations in organic solvents as compared to the random conformations. Our interpretation is supported by circular dichroism spectra of the peptides in the same solvent systems and by previously published structural data for the peptides. These results demonstrate the utility of MALDI-TOF as a method to monitor the H/D exchange chemistry of peptides and investigations of solution-phase conformations of biomolecules.

Entities: Chemical Gene

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Year: 1999 PMID： 10439510 DOI： 10.1016/S1044-0305(99)00040-9

Source DB: PubMed Journal: J Am Soc Mass Spectrom ISSN： 1044-0305 Impact factor: 3.109

48 in total

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Journal: Curr Opin Struct Biol Date: 1998-02 Impact factor: 6.809

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Journal: Int J Pept Protein Res Date: 1982-05

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Authors: S C Quay; C C Condie
Journal: Biochemistry Date: 1983-02-01 Impact factor: 3.162

11 in total

1. The structure of gas-phase bradykinin fragment 1-5 (RPPGF) ions: an ion mobility spectrometry and H/D exchange ion-molecule reaction chemistry study.

Authors: Holly A Sawyer; Joseph T Marini; Earle G Stone; Brandon T Ruotolo; Kent J Gillig; David H Russell
Journal: J Am Soc Mass Spectrom Date: 2005-06 Impact factor: 3.109

2. A mechanistic study of the H/D exchange reactions of protonated arginine and arginine-containing di- and tripeptides.

Authors: Yiqun Huang; Joe A Marini; John A McLean; Shane E Tichy; David H Russell
Journal: J Am Soc Mass Spectrom Date: 2009-07-30 Impact factor: 3.109

3. Hydrogen/deuterium exchange of hydrophobic peptides in model membranes by electrospray ionization mass spectrometry.

Authors: Raino K Hansen; R William Broadhurst; Paul C Skelton; Isaiah T Arkin
Journal: J Am Soc Mass Spectrom Date: 2002-12 Impact factor: 3.109

4. Nonresonant femtosecond laser vaporization of aqueous protein preserves folded structure.

Authors: John J Brady; Elizabeth J Judge; Robert J Levis
Journal: Proc Natl Acad Sci U S A Date: 2011-07-11 Impact factor: 11.205

5. Hydrogen-deuterium exchange at non-labile sites: a new reaction facet with broad implications for structural and dynamic determinations.

Authors: D R Reed; S R Kass
Journal: J Am Soc Mass Spectrom Date: 2001-11 Impact factor: 3.109

6. A Study of Ion-Neutral Collision Cross Section Values for Low Charge States of Peptides, Proteins, and Peptide/Protein Complexes.

Authors: Francisco A Fernandez-Lima; Ryan C Blase; David H Russell
Journal: Int J Mass Spectrom Date: 2010-12-01 Impact factor: 1.986

7. Factors that influence helical preferences for singly charged gas-phase peptide ions: the effects of multiple potential charge-carrying sites.

Authors: Janel R McLean; John A McLean; Zhaoxiang Wu; Christopher Becker; Lisa M Pérez; C Nick Pace; J Martin Scholtz; David H Russell
Journal: J Phys Chem B Date: 2010-01-21 Impact factor: 2.991

8. Hydrogen/deuterium exchange on yeast ATPase supramolecular protein complex analyzed at high sensitivity by MALDI mass spectrometry.

Authors: Alexis Nazabal; Michel Laguerre; Jean-Marie Schmitter; Jacques Vaillier; Stéphane Chaignepain; Jean Velours
Journal: J Am Soc Mass Spectrom Date: 2003-05 Impact factor: 3.109

9. Steroid and protein ligand binding to cytochrome P450 46A1 as assessed by hydrogen-deuterium exchange and mass spectrometry.

Authors: Wei-Li Liao; Nathan G Dodder; Natalia Mast; Irina A Pikuleva; Illarion V Turko
Journal: Biochemistry Date: 2009-05-19 Impact factor: 3.162

10. The methanol-induced conformational transitions of beta-lactoglobulin, cytochrome c, and ubiquitin at low pH: a study by electrospray ionization mass spectrometry.

Authors: K R Babu; A Moradian; D J Douglas
Journal: J Am Soc Mass Spectrom Date: 2001-03 Impact factor: 3.262