Literature DB >> 10432308

Novel method for evaluation of the oligomeric structure of membrane proteins.

M Ramjeesingh1, L J Huan, E Garami, C E Bear.   

Abstract

Assessment of the quaternary structure of membrane proteins by PAGE has been problematic owing to their relatively poor solubility in non-dissociative detergents. Here we report that several membrane proteins can be readily solubilized in their native quaternary structure with the use of the detergent perfluoro-octanoic acid (PFO). Further, PFO can be used with PAGE, thereby providing a novel, accessible tool with which to assess the molecular mass of homo-multimeric protein complexes.

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Year:  1999        PMID： 10432308      PMCID： PMC1220444     

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  The structure of the potassium channel: molecular basis of K+ conduction and selectivity.

Authors:  D A Doyle; J Morais Cabral; R A Pfuetzner; A Kuo; J M Gulbis; S L Cohen; B T Chait; R MacKinnon
Journal:  Science       Date:  1998-04-03       Impact factor: 47.728

Review 2.  Which GABAA-receptor subtypes really occur in the brain?

Authors:  R M McKernan; P J Whiting
Journal:  Trends Neurosci       Date:  1996-04       Impact factor: 13.837

3.  A novel procedure for the efficient purification of the cystic fibrosis transmembrane conductance regulator (CFTR).

Authors:  M Ramjeesingh; C Li; E Garami; L J Huan; M Hewryk; Y Wang; K Galley; C E Bear
Journal:  Biochem J       Date:  1997-10-01       Impact factor: 3.857

4.  The three-dimensional structure of aquaporin-1.

Authors:  T Walz; T Hirai; K Murata; J B Heymann; K Mitsuoka; Y Fujiyoshi; B L Smith; P Agre; A Engel
Journal:  Nature       Date:  1997-06-05       Impact factor: 49.962

5.  Self-association of Band 3, the human erythrocyte anion exchanger, in detergent solution.

Authors:  J W Vince; V E Sarabia; R A Reithmeier
Journal:  Biochim Biophys Acta       Date:  1997-06-12

6.  Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis.

Authors:  H Schägger; W A Cramer; G von Jagow
Journal:  Anal Biochem       Date:  1994-03       Impact factor: 3.365

7.  The potential of fluorinated surfactants in membrane biochemistry.

Authors:  F H Shepherd; A Holzenburg
Journal:  Anal Biochem       Date:  1995-01-01       Impact factor: 3.365

8.  Determination of the subunit stoichiometry of an inwardly rectifying potassium channel.

Authors:  J Yang; Y N Jan; L Y Jan
Journal:  Neuron       Date:  1995-12       Impact factor: 17.173

9.  Oligomerization state of water channels and glycerol facilitators. Involvement of loop E.

Authors:  V Lagrée; A Froger; S Deschamps; I Pellerin; C Delamarche; G Bonnec; J Gouranton; D Thomas; J F Hubert
Journal:  J Biol Chem       Date:  1998-12-18       Impact factor: 5.157

10.  Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form.

Authors:  H Schägger; G von Jagow
Journal:  Anal Biochem       Date:  1991-12       Impact factor: 3.365
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  64 in total

1.  Dimerization of human XPA and formation of XPA2-RPA protein complex.

Authors:  Zheng-guan Yang; Yang Liu; Leslie Y Mao; Jian-Ting Zhang; Yue Zou
Journal:  Biochemistry       Date:  2002-10-29       Impact factor: 3.162

2.  Expression, solubilization, and biochemical characterization of the tight junction transmembrane protein claudin-4.

Authors:  Laura L Mitic; Vinzenz M Unger; James Melvin Anderson
Journal:  Protein Sci       Date:  2003-02       Impact factor: 6.725

Review 3.  Transferring knowledge towards understanding the pore stabilizing variations in K(+) channels: pore stability in K(+) channels.

Authors:  Mobeen Raja; Nick K Olrichs; Elisabeth Vales; Hildgund Schrempf
Journal:  J Bioenerg Biomembr       Date:  2012-02       Impact factor: 2.945

4.  3-D structural and functional characterization of the purified KATP channel complex Kir6.2-SUR1.

Authors:  Michael V Mikhailov; Jeff D Campbell; Heidi de Wet; Kenju Shimomura; Brittany Zadek; Richard F Collins; Mark S P Sansom; Robert C Ford; Frances M Ashcroft
Journal:  EMBO J       Date:  2005-11-24       Impact factor: 11.598

5.  Oligomerization of the fifth transmembrane domain from the adenosine A2A receptor.

Authors:  Damien Thévenin; Tzvetana Lazarova; Matthew F Roberts; Clifford R Robinson
Journal:  Protein Sci       Date:  2005-06-29       Impact factor: 6.725

6.  Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles.

Authors:  Peter Teriete; Carla M Franzin; Jungyuen Choi; Francesca M Marassi
Journal:  Biochemistry       Date:  2007-05-19       Impact factor: 3.162

7.  Nuclear magnetic resonance structure of the N-terminal domain of nonstructural protein 3 from the severe acute respiratory syndrome coronavirus.

Authors:  Pedro Serrano; Margaret A Johnson; Marcius S Almeida; Reto Horst; Torsten Herrmann; Jeremiah S Joseph; Benjamin W Neuman; Vanitha Subramanian; Kumar S Saikatendu; Michael J Buchmeier; Raymond C Stevens; Peter Kuhn; Kurt Wüthrich
Journal:  J Virol       Date:  2007-08-29       Impact factor: 5.103

8.  Coexpression and heteromerization of two neuronal K-Cl cotransporter isoforms in neonatal brain.

Authors:  Pavel Uvarov; Anastasia Ludwig; Marika Markkanen; Shetal Soni; Christian A Hübner; Claudio Rivera; Matti S Airaksinen
Journal:  J Biol Chem       Date:  2009-03-23       Impact factor: 5.157

9.  Structure-function relationships of the outer membrane translocon Wza investigated by cryo-electron microscopy and mutagenesis.

Authors:  Robert C Ford; Anne L Brunkan-LaMontagne; Richard F Collins; Bradley R Clarke; Robert Harris; James H Naismith; Chris Whitfield
Journal:  J Struct Biol       Date:  2009-02-21       Impact factor: 2.867

10.  Latrophilin fragments behave as independent proteins that associate and signal on binding of LTX(N4C).

Authors:  Kirill E Volynski; John-Paul Silva; Vera G Lelianova; M Atiqur Rahman; Colin Hopkins; Yuri A Ushkaryov
Journal:  EMBO J       Date:  2004-10-14       Impact factor: 11.598
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