Literature DB >> 10427069

Quantification of the flavonoid-degrading bacterium Eubacterium ramulus in human fecal samples with a species-specific oligonucleotide hybridization probe.

R Simmering1, B Kleessen, M Blaut.   

Abstract

To investigate the occurrence of the flavonoid-degrading bacterium Eubacterium ramulus in the human intestinal tract, an oligonucleotide probe designated S-S-E.ram-0997-a-A-18 was designed and validated, with over 90 bacterial strains representing the dominant described human fecal flora. Application of S-S-E. ram-0997-a-A-18 to fecal samples from 20 subjects indicated the presence of E. ramulus in each individual tested in numbers from 4.4 x 10(7) to 2.0 x 10(9) cells/g of fecal dry mass. Six fecal E. ramulus isolates were recognized by S-S-E.ram-0997-a-A-18 but exhibited different band patterns when analyzed by randomly amplified polymorphic DNA.

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Year:  1999        PMID： 10427069      PMCID： PMC91554     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  30 in total

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Authors:  M P Bryant
Journal:  Am J Clin Nutr       Date:  1972-12       Impact factor: 7.045

2.  3-Aminopropyltriethoxysilane (APES): a new advance in section adhesion.

Authors:  P H Maddox; D Jenkins
Journal:  J Clin Pathol       Date:  1987-10       Impact factor: 3.411

3.  Disposition of quercetin in man after single oral and intravenous doses.

Authors:  R Gugler; M Leschik; H J Dengler
Journal:  Eur J Clin Pharmacol       Date:  1975-12-19       Impact factor: 2.953

4.  Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes.

Authors:  A H Franks; H J Harmsen; G C Raangs; G J Jansen; F Schut; G W Welling
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

5.  Mutagenic activity of quercetin and related compounds.

Authors:  L F Bjeldanes; G W Chang
Journal:  Science       Date:  1977-08-05       Impact factor: 47.728

6.  Identification of flavanone metabolites in rat urine by combined gas-liquid chromatography and mass spectrometry.

Authors:  N P Das; K N Scott; J H Duncan
Journal:  Biochem J       Date:  1973-12       Impact factor: 3.857

7.  Tyrosine protein kinase activity in the DMBA-induced rat mammary tumor: inhibition by quercetin.

Authors:  J Levy; I Teuerstein; M Marbach; S Radian; Y Sharoni
Journal:  Biochem Biophys Res Commun       Date:  1984-09-28       Impact factor: 3.575

8.  Human fecal flora: the normal flora of 20 Japanese-Hawaiians.

Authors:  W E Moore; L V Holdeman
Journal:  Appl Microbiol       Date:  1974-05

9.  Anaerobic transformation of quercetin-3-glucoside by bacteria from the human intestinal tract.

Authors:  H Schneider; A Schwiertz; M D Collins; M Blaut
Journal:  Arch Microbiol       Date:  1999-01       Impact factor: 2.552

10.  Quercetin inhibits the action of 12-O-tetradecanoylphorbol-13-acetate, a tumor promoter.

Authors:  H Nishino; A Nishino; A Iwashima; K Tanaka; T Matsuura
Journal:  Oncology       Date:  1984       Impact factor: 2.935
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  15 in total

1.  Extensive set of 16S rRNA-based probes for detection of bacteria in human feces.

Authors:  Hermie J M Harmsen; Gerwin C Raangs; Tao He; John E Degener; Gjalt W Welling
Journal:  Appl Environ Microbiol       Date:  2002-06       Impact factor: 4.792

2.  Cloning and expression of a phloretin hydrolase gene from Eubacterium ramulus and characterization of the recombinant enzyme.

Authors:  Lilian Schoefer; Annett Braune; Michael Blaut
Journal:  Appl Environ Microbiol       Date:  2004-10       Impact factor: 4.792

3.  Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale.

Authors:  Rustam I Aminov; Alan W Walker; Sylvia H Duncan; Hermie J M Harmsen; Gjalt W Welling; Harry J Flint
Journal:  Appl Environ Microbiol       Date:  2006-09       Impact factor: 4.792

Review 4.  Proposed model: mechanisms of immunomodulation induced by probiotic bacteria.

Authors:  C Maldonado Galdeano; A de Moreno de LeBlanc; G Vinderola; M E Bibas Bonet; G Perdigón
Journal:  Clin Vaccine Immunol       Date:  2007-03-14

5.  Quantification of different Eubacterium spp. in human fecal samples with species-specific 16S rRNA-targeted oligonucleotide probes.

Authors:  A Schwiertz; G Le Blay; M Blaut
Journal:  Appl Environ Microbiol       Date:  2000-01       Impact factor: 4.792

6.  Degradation of quercetin and luteolin by Eubacterium ramulus.

Authors:  A Braune; M Gütschow; W Engst; M Blaut
Journal:  Appl Environ Microbiol       Date:  2001-12       Impact factor: 4.792

Review 7.  Bacterial species involved in the conversion of dietary flavonoids in the human gut.

Authors:  Annett Braune; Michael Blaut
Journal:  Gut Microbes       Date:  2016-03-10

8.  An NADH-Dependent Reductase from Eubacterium ramulus Catalyzes the Stereospecific Heteroring Cleavage of Flavanones and Flavanonols.

Authors:  Annett Braune; Michael Gütschow; Michael Blaut
Journal:  Appl Environ Microbiol       Date:  2019-09-17       Impact factor: 4.792

9.  Oligonucleotide probes that detect quantitatively significant groups of butyrate-producing bacteria in human feces.

Authors:  Georgina L Hold; Andreas Schwiertz; Rustam I Aminov; Michael Blaut; Harry J Flint
Journal:  Appl Environ Microbiol       Date:  2003-07       Impact factor: 4.792

Review 10.  Gut microbiome diversity in acute infective and chronic inflammatory gastrointestinal diseases in North India.

Authors:  Saurabh Kedia; Ritika Rampal; Jaishree Paul; Vineet Ahuja
Journal:  J Gastroenterol       Date:  2016-03-19       Impact factor: 7.527
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