Literature DB >> 10423381

Triple-resonance experiments for correlation of H5 and exchangeable pyrimidine base hydrogens in (13)C,(15)N-labeled RNA.

J Wöhnert1, R Ramachandran, M Görlach, L R Brown.   

Abstract

Triple-resonance two-dimensional H5(C5C4N)H experiments are described that provide through-bond H5 to imino/amino connectivities in uridines and cytidines in (13)C, (15)N-labeled RNAs. The experiments employ selective INEPT steps for transferring magnetization from the H5 hydrogens through the intervening C5, C4, and N3/N4 nuclei to the imino/amino hydrogens. The improved sensitivity of these experiments for assignments in a large 43-nucleotide RNA is demonstrated. Copyright 1999 Academic Press.

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Year:  1999        PMID: 10423381     DOI: 10.1006/jmre.1999.1797

Source DB:  PubMed          Journal:  J Magn Reson        ISSN: 1090-7807            Impact factor:   2.229


  17 in total

1.  Long-range imino proton-13C J-couplings and the through-bond correlation of imino and non-exchangeable protons in unlabeled DNA.

Authors:  A T Phan
Journal:  J Biomol NMR       Date:  2000-02       Impact factor: 2.835

2.  Redor in IS1S2 systems.

Authors:  J Leppert; B Heise; R Ramachandran
Journal:  J Biomol NMR       Date:  2000-10       Impact factor: 2.835

3.  NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor.

Authors:  Christophe Maris; James Masse; Ann Chester; Naveenan Navaratnam; Frédéric H-T Allain
Journal:  RNA       Date:  2005-02       Impact factor: 4.942

4.  1H-1H correlations across N-H...N hydrogen bonds in nucleic acids.

Authors:  A Majumdar; Y Gosser; D J Patel
Journal:  J Biomol NMR       Date:  2001-12       Impact factor: 2.835

5.  Structural determinants for ligand capture by a class II preQ1 riboswitch.

Authors:  Mijeong Kang; Catherine D Eichhorn; Juli Feigon
Journal:  Proc Natl Acad Sci U S A       Date:  2014-01-27       Impact factor: 11.205

6.  Structural conservation in the template/pseudoknot domain of vertebrate telomerase RNA from teleost fish to human.

Authors:  Yaqiang Wang; Joseph D Yesselman; Qi Zhang; Mijeong Kang; Juli Feigon
Journal:  Proc Natl Acad Sci U S A       Date:  2016-08-16       Impact factor: 11.205

Review 7.  Applications of NMR to structure determination of RNAs large and small.

Authors:  Ravi P Barnwal; Fan Yang; Gabriele Varani
Journal:  Arch Biochem Biophys       Date:  2017-06-16       Impact factor: 4.013

8.  Experiments for correlating quaternary carbons in RNA bases.

Authors:  Radovan Fiala; Markéta L Munzarová; Vladimír Sklenár
Journal:  J Biomol NMR       Date:  2004-08       Impact factor: 2.835

9.  Triple resonance experiments for the simultaneous correlation of H6/H5 and exchangeable protons of pyrimidine nucleotides in 13C,15N-labeled RNA applicable to larger RNA molecules.

Authors:  Jens Wöhnert; Matthias Görlach; Harald Schwalbe
Journal:  J Biomol NMR       Date:  2003-05       Impact factor: 2.835

10.  Solution structures of stem-loop RNAs that bind to the two N-terminal RNA-binding domains of nucleolin.

Authors:  L David Finger; Lukas Trantirek; Carina Johansson; Juli Feigon
Journal:  Nucleic Acids Res       Date:  2003-11-15       Impact factor: 16.971

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