| Literature DB >> 10423381 |
J Wöhnert1, R Ramachandran, M Görlach, L R Brown.
Abstract
Triple-resonance two-dimensional H5(C5C4N)H experiments are described that provide through-bond H5 to imino/amino connectivities in uridines and cytidines in (13)C, (15)N-labeled RNAs. The experiments employ selective INEPT steps for transferring magnetization from the H5 hydrogens through the intervening C5, C4, and N3/N4 nuclei to the imino/amino hydrogens. The improved sensitivity of these experiments for assignments in a large 43-nucleotide RNA is demonstrated. Copyright 1999 Academic Press.Entities:
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Year: 1999 PMID: 10423381 DOI: 10.1006/jmre.1999.1797
Source DB: PubMed Journal: J Magn Reson ISSN: 1090-7807 Impact factor: 2.229