M Okada1, C Bothin, K Kanazawa, T Midtvedt. 1. Department of Medical Microbial Ecology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden.
Abstract
BACKGROUND: The beneficial effects of the normal intestinal flora on wound healing in the skin have already been confirmed, and this study attempted to elucidate the influence of the intestinal flora on the healing process in intestinal anastomoses. METHODS: Five groups of rats were studied: germ-free, conventional, monocontaminated with Lactobacillus acidophilus La5 or Escherichia coli X7 and ex-germ-free (conventionalized). All animals underwent ileal and colonic resections followed by anastomoses. Seven days later they were killed and the bursting pressure and hydroxyproline concentration of the anastomoses were measured. The microbiological status of the animals was confirmed weekly. RESULTS: No bacteria were detected in the germ-free rats and no other bacteria were found in the monocontaminated animals. Conventional rats had a significantly higher anastomotic bursting pressure both in the ileum compared with rats monocontaminated with L. acidophilus, and in the colon compared with germ-free rats. The ex-germ-free rats also showed a significantly higher bursting pressure than germ-free animals and rats monocontaminated with either L. acidophilus or E. coli in the ileum and colon. CONCLUSION: The presence of the intestinal flora enhanced the healing of intestinal anastomoses. The data suggest that this effect depends on differences in the types of bacteria in the intestine.
BACKGROUND: The beneficial effects of the normal intestinal flora on wound healing in the skin have already been confirmed, and this study attempted to elucidate the influence of the intestinal flora on the healing process in intestinal anastomoses. METHODS: Five groups of rats were studied: germ-free, conventional, monocontaminated with Lactobacillus acidophilus La5 or Escherichia coli X7 and ex-germ-free (conventionalized). All animals underwent ileal and colonic resections followed by anastomoses. Seven days later they were killed and the bursting pressure and hydroxyproline concentration of the anastomoses were measured. The microbiological status of the animals was confirmed weekly. RESULTS: No bacteria were detected in the germ-free rats and no other bacteria were found in the monocontaminated animals. Conventional rats had a significantly higher anastomotic bursting pressure both in the ileum compared with rats monocontaminated with L. acidophilus, and in the colon compared with germ-free rats. The ex-germ-free rats also showed a significantly higher bursting pressure than germ-free animals and rats monocontaminated with either L. acidophilus or E. coli in the ileum and colon. CONCLUSION: The presence of the intestinal flora enhanced the healing of intestinal anastomoses. The data suggest that this effect depends on differences in the types of bacteria in the intestine.
Authors: Mari Nanna Oines; Peter-Martin Krarup; Lars Nannestad Jorgensen; Magnus Sven Agren Journal: World J Gastroenterol Date: 2014-09-21 Impact factor: 5.742
Authors: Miquell O Miller; Purna C Kashyap; Sarah L Becker; Ryan M Thomas; Richard A Hodin; George Miller; Mautin Hundeyin; Smruti Pushalkar; Deirdre Cohen; Deepak Saxena; Benjamin D Shogan; Gareth J Morris-Stiff Journal: J Gastrointest Surg Date: 2021-07 Impact factor: 3.452
Authors: Richard A Jacobson; Kiedo Wienholts; Ashley J Williamson; Sara Gaines; Sanjiv Hyoju; Harry van Goor; Alexander Zaborin; Benjamin D Shogan; Olga Zaborina; John C Alverdy Journal: Am J Physiol Gastrointest Liver Physiol Date: 2019-10-11 Impact factor: 4.052
Authors: N Regenet; P Pessaux; S Hennekinne; E Lermite; J J Tuech; O Brehant; J P Arnaud Journal: Int J Colorectal Dis Date: 2003-08-09 Impact factor: 2.571