| Literature DB >> 10407164 |
J A Brannigan1, S R Ashford, A J Doherty, D J Timson, D B Wigley.
Abstract
The gene for DNA ligase (EC 6.5.1.2) from thermophilic bacterium Bacillus stearothermophilus NCA1503 has been cloned and the complete nucleotide sequence determined. The ligase gene encodes a protein 670 amino acids in length. The gene was overexpressed in Escherichia coli and the enzyme has been purified to homogeneity. Preliminary characterisation confirms that it is a thermostable, NAD(+)-dependent DNA ligase.Entities:
Mesh：
Substances：
Year: 1999 PMID： 10407164 DOI： 10.1016/s0167-4838(99)00122-3
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002