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Literature DB >> 10393322

Molecular cloning and functional expression of the human Golgi UDP-N-acetylglucosamine transporter.

N Ishida¹, S Yoshioka, Y Chiba, M Takeuchi, M Kawakita.

Abstract

We have cloned the human UDP-N-acetylglucosamine (UDP-GlcNAc) transporter cDNA, which was recognized through a homology search in the expressed sequence tags database (dbEST) based on its similarity to the human UDP-galactose transporter. The chromosomal location of the UDP-GlcNAc transporter gene was assigned to chromosome 1p21 by fluorescence in situ hybridization (FISH). The transporter was expressed ubiquitously in every tissue so far examined. Expression of the transporter cDNA in CHO-K1 cells in its native and in a C-terminally HA-tagged form indicated that the human UDP-GlcNAc transporter was localized in the Golgi apparatus. The membrane vesicles prepared from yeast cells expressing the cDNA product exhibited UDP-GlcNAc-specific transporting activity. Comparison among UDP-galactose, CMP-sialic acid, and UDP-GlcNAc transporters from several organisms enabled us to identify residues highly conserved among the transporters and residues specific for each group of transporters.

Entities: Chemical Gene Species

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Year: 1999 PMID： 10393322 DOI： 10.1093/oxfordjournals.jbchem.a022437

Source DB: PubMed Journal: J Biochem ISSN： 0021-924X Impact factor: 3.387

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Cited

19 in total

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2. The Concise Guide to PHARMACOLOGY 2013/14: transporters.

Authors: Stephen P H Alexander; Helen E Benson; Elena Faccenda; Adam J Pawson; Joanna L Sharman; Michael Spedding; John A Peters; Anthony J Harmar
Journal: Br J Pharmacol Date: 2013-12 Impact factor: 8.739

3. Conserved Glu-47 and Lys-50 residues are critical for UDP-N-acetylglucosamine/UMP antiport activity of the mouse Golgi-associated transporter Slc35a3.

Authors: M Agustina Toscanini; M Belén Favarolo; F Luis Gonzalez Flecha; Berit Ebert; Carsten Rautengarten; Luis M Bredeston
Journal: J Biol Chem Date: 2019-05-22 Impact factor: 5.157

4. Isolation, sequence identification and tissue expression distribution of three novel porcine genes--RAB14, S35A3 and ITM2A.

Authors: G Y Liu; C R Ge; X Zhang; S Z Gao
Journal: Mol Biol Rep Date: 2007-03-23 Impact factor: 2.316

5. Impaired O-linked N-acetylglucosaminylation in the endoplasmic reticulum by mutated epidermal growth factor (EGF) domain-specific O-linked N-acetylglucosamine transferase found in Adams-Oliver syndrome.

Authors: Mitsutaka Ogawa; Shogo Sawaguchi; Takami Kawai; Daita Nadano; Tsukasa Matsuda; Hirokazu Yagi; Koichi Kato; Koichi Furukawa; Tetsuya Okajima
Journal: J Biol Chem Date: 2014-12-08 Impact factor: 5.157

6. Biosynthesis of GlcNAc-rich N- and O-glycans in the Golgi apparatus does not require the nucleotide sugar transporter SLC35A3.

Authors: Bozena Szulc; Paulina Sosicka; Dorota Maszczak-Seneczko; Edyta Skurska; Auhen Shauchuk; Teresa Olczak; Hudson H Freeze; Mariusz Olczak
Journal: J Biol Chem Date: 2020-09-16 Impact factor: 5.157

10. UDP-N-acetylglucosamine transporter (SLC35A3) regulates biosynthesis of highly branched N-glycans and keratan sulfate.

Authors: Dorota Maszczak-Seneczko; Paulina Sosicka; Teresa Olczak; Piotr Jakimowicz; Michał Majkowski; Mariusz Olczak
Journal: J Biol Chem Date: 2013-06-13 Impact factor: 5.157