Literature DB >> 10383983

Identification and expression of the genes encoding a reactivating factor for adenosylcobalamin-dependent glycerol dehydratase.

T Tobimatsu1, H Kajiura, M Yunoki, M Azuma, T Toraya.   

Abstract

Adenosylcobalamin-dependent glycerol dehydratase undergoes inactivation by glycerol, the physiological substrate, during catalysis. In permeabilized cells of Klebsiella pneumoniae, the inactivated enzyme is reactivated in the presence of ATP, Mg2+, and adenosylcobalamin. We identified the two open reading frames as the genes for a reactivating factor for glycerol dehydratase and designated them gdrA and gdrB. The reactivation of the inactivated glycerol dehydratase by the gene products was confirmed in permeabilized recombinant Escherichia coli cells coexpressing GdrA and GdrB proteins with glycerol dehydratase.

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Year:  1999        PMID： 10383983      PMCID： PMC93905     

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

1.  A cobamide-requiring glycerol dehydrase from an acrolein-forming Lactobacillus.

Authors:  K L SMILEY; M SOBOLOV
Journal:  Arch Biochem Biophys       Date:  1962-06       Impact factor: 4.013

2.  Substrate specificity of coenzyme B12-dependent diol dehydrase: glycerol as both a good substrate and a potent inactivator.

Authors:  T Toraya; T Shirakashi; T Kosuga; S Fukui
Journal:  Biochem Biophys Res Commun       Date:  1976-03-22       Impact factor: 3.575

3.  Study of the mechanism of action of adenosylcobalamindependent glycerol dehydratase from Aerobacter aerogenes. II. The inactivation kinetics of glycerol dehydratase complexes with adenosylobalamin and its analogs.

Authors:  A A Poznanskaya; M I Yakusheva; V A Yakovlev
Journal:  Biochim Biophys Acta       Date:  1977-09-15

4.  Determination of glycerol dehydratase activity by the coupled enzymic method.

Authors:  M I Yakusheva; A A Malahov; A A Poznanskaya; V A Yakovlev
Journal:  Anal Biochem       Date:  1974-07       Impact factor: 3.365

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Distribution of coenzyme B12-dependent diol dehydratase and glycerol dehydratase in selected genera of Enterobacteriaceae and Propionibacteriaceae.

Authors:  T Toraya; S Kuno; S Fukui
Journal:  J Bacteriol       Date:  1980-03       Impact factor: 3.490

7.  Enzymic conversion of glycerol into beta-hydroxy-propionaldehyde in a cell-free extract from Aerobacter aerogenes.

Authors:  J Pawelkiewicz; B Zagalak
Journal:  Acta Biochim Pol       Date:  1965       Impact factor: 2.149

8.  Mechanism of action of adenosylcobalamin: glycerol and other substrate analogues as substrates and inactivators for propanediol dehydratase--kinetics, stereospecificity, and mechanism.

Authors:  W W Bachovchin; R G Eagar; K W Moore; J H Richards
Journal:  Biochemistry       Date:  1977-03-22       Impact factor: 3.162

9.  Studies on the mechanism of the adenosylcobalamin-dependent diol dehydrase reaction by the use of analogs of the coenzyme.

Authors:  T Toraya; K Ushio; S Fukui; P C Hogenkamp
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

10.  In situ reactivation of glycerol-inactivated coenzyme B12-dependent enzymes, glycerol dehydratase and diol dehydratase.

Authors:  S Honda; T Toraya; S Fukui
Journal:  J Bacteriol       Date:  1980-09       Impact factor: 3.490
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  6 in total

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Authors:  Chiranjit Chowdhury; Sharmistha Sinha; Sunny Chun; Todd O Yeates; Thomas A Bobik
Journal:  Microbiol Mol Biol Rev       Date:  2014-09       Impact factor: 11.056

2.  Influence of dhaT mutation of K. pneumoniae on 1,3-propanediol fermentation.

Authors:  Yun-Zhen Xu; Ru-Chun Wu; Zong-Ming Zheng; De-Hua Liu
Journal:  World J Microbiol Biotechnol       Date:  2010-11-09       Impact factor: 3.312

3.  Evidence that a B12-adenosyl transferase is encoded within the ethanolamine operon of Salmonella enterica.

Authors:  David E Sheppard; Joseph T Penrod; Thomas Bobik; Eric Kofoid; John R Roth
Journal:  J Bacteriol       Date:  2004-11       Impact factor: 3.490

4.  Identification of a reactivating factor for adenosylcobalamin-dependent ethanolamine ammonia lyase.

Authors:  Koichi Mori; Reiko Bando; Naoki Hieda; Tetsuo Toraya
Journal:  J Bacteriol       Date:  2004-10       Impact factor: 3.490

5.  Crystallization and preliminary X-ray analysis of molecular chaperone-like diol dehydratase-reactivating factor in ADP-bound and nucleotide-free forms.

Authors:  Koichi Mori; Naoki Hieda; Mamoru Yamanishi; Naoki Shibata; Tetsuo Toraya
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-06-01

Review 6.  Key enzymes catalyzing glycerol to 1,3-propanediol.

Authors:  Wei Jiang; Shizhen Wang; Yuanpeng Wang; Baishan Fang
Journal:  Biotechnol Biofuels       Date:  2016-03-10       Impact factor: 6.040
  6 in total

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