Literature DB >> 10364365

Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1-infected individuals on prolonged effective antiretroviral therapy.

S R Lewin1, M Vesanen, L Kostrikis, A Hurley, M Duran, L Zhang, D D Ho, M Markowitz.   

Abstract

We have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR and molecular beacons. Individuals were classified as either well suppressed (WS) or partially suppressed, based on longitudinal measurements of plasma HIV-1 RNA. The proportion of individuals with US mRNA undetectable over time was significantly higher among WS individuals; however, 30% of WS subjects still had detectable US mRNA after 24 months of effective antiviral therapy.

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Year:  1999        PMID： 10364365      PMCID： PMC112674          DOI： 10.1128/JVI.73.7.6099-6103.1999

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  31 in total

1.  Real time quantitative PCR.

Authors:  C A Heid; J Stevens; K J Livak; P M Williams
Journal:  Genome Res       Date:  1996-10       Impact factor: 9.043

2.  Dynamics and modulation of human immunodeficiency virus type 1 transcripts in vitro and in vivo.

Authors:  P Bagnarelli; A Valenza; S Menzo; R Sampaolesi; P E Varaldo; L Butini; M Montroni; C F Perno; S Aquaro; D Mathez; J Leibowitch; C Balotta; M Clementi
Journal:  J Virol       Date:  1996-11       Impact factor: 5.103

3.  Analysis of human immunodeficiency virus type 1 mRNA splicing patterns during disease progression in peripheral blood mononuclear cells from infected individuals.

Authors:  M J Saltarelli; E Hadziyannis; C E Hart; J V Harrison; B K Felber; T J Spira; G N Pavlakis
Journal:  AIDS Res Hum Retroviruses       Date:  1996-10-10       Impact factor: 2.205

4.  Human immunodeficiency virus type 1 cellular RNA load and splicing patterns predict disease progression in a longitudinally studied cohort.

Authors:  N L Michael; T Mo; A Merzouki; M O'Shaughnessy; C Oster; D S Burke; R R Redfield; D L Birx; S A Cassol
Journal:  J Virol       Date:  1995-03       Impact factor: 5.103

5.  Kinetics of expression of multiply spliced RNA in early human immunodeficiency virus type 1 infection of lymphocytes and monocytes.

Authors:  M E Klotman; S Kim; A Buchbinder; A DeRossi; D Baltimore; F Wong-Staal
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-01       Impact factor: 11.205

6.  HIV-1 messenger RNA in peripheral blood mononuclear cells as an early marker of risk for progression to AIDS.

Authors:  K Saksela; C E Stevens; P Rubinstein; P E Taylor; D Baltimore
Journal:  Ann Intern Med       Date:  1995-11-01       Impact factor: 25.391

7.  Expression kinetics and subcellular localization of HIV-1 regulatory proteins Nef, Tat and Rev in acutely and chronically infected lymphoid cell lines.

Authors:  A Ranki; A Lagerstedt; V Ovod; E Aavik; K J Krohn
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

8.  Changes in the viral mRNA expression pattern correlate with a rapid rate of CD4+ T-cell number decline in human immunodeficiency virus type 1-infected individuals.

Authors:  M R Furtado; L A Kingsley; S M Wolinsky
Journal:  J Virol       Date:  1995-04       Impact factor: 5.103

9.  In vivo fate of HIV-1-infected T cells: quantitative analysis of the transition to stable latency.

Authors:  T W Chun; D Finzi; J Margolick; K Chadwick; D Schwartz; R F Siliciano
Journal:  Nat Med       Date:  1995-12       Impact factor: 53.440

10.  Quiescent T lymphocytes as an inducible virus reservoir in HIV-1 infection.

Authors:  M I Bukrinsky; T L Stanwick; M P Dempsey; M Stevenson
Journal:  Science       Date:  1991-10-18       Impact factor: 63.714
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  88 in total

1.  Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples.

Authors:  Ying Fang; Wai-Hong Wu; Jessica L Pepper; Jill L Larsen; Salvatore A E Marras; Eric A Nelson; William B Epperson; Jane Christopher-Hennings
Journal:  J Clin Microbiol       Date:  2002-01       Impact factor: 5.948

Review 2.  Real-time PCR in virology.

Authors:  Ian M Mackay; Katherine E Arden; Andreas Nitsche
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

3.  Principles of quantitation of viral loads using nucleic acid sequence-based amplification in combination with homogeneous detection using molecular beacons.

Authors:  Jos J A M Weusten; Wim M Carpay; Tom A M Oosterlaken; Martien C A van Zuijlen; Paul A van de Wiel
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

4.  Rapid detection of herpes simplex virus DNA in genital ulcers by real-time PCR using SYBR green I dye as the detection signal.

Authors:  Carmen Aldea; Carmen P Alvarez; Lola Folgueira; Rafael Delgado; Joaquín R Otero
Journal:  J Clin Microbiol       Date:  2002-03       Impact factor: 5.948

5.  Quantification of human immunodeficiency virus type 1 proviral DNA by the TaqMan real-time PCR assay.

Authors:  Zhibing Yun; Eva Fredriksson; Anders Sönnerborg
Journal:  J Clin Microbiol       Date:  2002-10       Impact factor: 5.948

6.  Hematopoietic precursor cells isolated from patients on long-term suppressive HIV therapy did not contain HIV-1 DNA.

Authors:  Lina Josefsson; Susanne Eriksson; Elizabeth Sinclair; Terence Ho; Maudi Killian; Lorrie Epling; Wei Shao; Bradley Lewis; Peter Bacchetti; Lisa Loeb; Jeff Custer; Lauren Poole; Frederick M Hecht; Sarah Palmer
Journal:  J Infect Dis       Date:  2012-04-25       Impact factor: 5.226

7.  Evidence for human immunodeficiency virus type 1 replication in vivo in CD14(+) monocytes and its potential role as a source of virus in patients on highly active antiretroviral therapy.

Authors:  Tuofu Zhu; David Muthui; Sarah Holte; David Nickle; Feng Feng; Scott Brodie; Yon Hwangbo; James I Mullins; Lawrence Corey
Journal:  J Virol       Date:  2002-01       Impact factor: 5.103

8.  Myeloid Dendritic Cells Induce HIV Latency in Proliferating CD4+ T Cells.

Authors:  Nitasha A Kumar; Renee M van der Sluis; Talia Mota; Rachel Pascoe; Vanessa A Evans; Sharon R Lewin; Paul U Cameron
Journal:  J Immunol       Date:  2018-07-20       Impact factor: 5.422

9.  Productive infection maintains a dynamic steady state of residual viremia in human immunodeficiency virus type 1-infected persons treated with suppressive antiretroviral therapy for five years.

Authors:  Diane V Havlir; Matthew C Strain; Mario Clerici; Caroline Ignacio; Daria Trabattoni; Pasquale Ferrante; Joseph K Wong
Journal:  J Virol       Date:  2003-10       Impact factor: 5.103

10.  Development of a LightCycler PCR assay for detection and quantification of Aspergillus fumigatus DNA in clinical samples from neutropenic patients.

Authors:  Birgit Spiess; Dieter Buchheidt; Corinna Baust; Heyko Skladny; Wolfgang Seifarth; Udo Zeilfelder; Christine Leib-Mösch; Handan Mörz; Rüdiger Hehlmann
Journal:  J Clin Microbiol       Date:  2003-05       Impact factor: 5.948
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