Literature DB >> 10347085

High-efficiency transformation of Rhizobium leguminosarum by electroporation.

B Garg1, R C Dogra, P K Sharma.   

Abstract

Electrotransformation of Rhizobium leguminosarum was successfully carried out with a 15.1-kb plasmid, pMP154 (Cmr), containing a nodABC-lacZ fusion by electroporation. The maximum transformation efficiency, 10(8) transformants/microg of DNA, was achieved at a field strength of 14 kV/cm with a pulse of 7.3 ms (186 Omega). The number of transformants was found to increase with increasing cell density, with no sign of saturation. In relation to DNA dosage, the maximum transformation efficiency (5.8 x 10(8) transformants/microg of DNA) was obtained with 0.5 microg of DNA/ml of cell suspension, and a further increase in the DNA concentration resulted in a decline in transformation efficiency.

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Year:  1999        PMID： 10347085      PMCID： PMC91420          DOI： 10.1128/AEM.65.6.2802-2804.1999

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

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Journal:  Gene       Date:  1990-05-31       Impact factor: 3.688

5.  Efficient DNA transformation of Bradyrhizobium japonicum by electroporation.

Authors:  D R Hattermann; G Stacey
Journal:  Appl Environ Microbiol       Date:  1990-04       Impact factor: 4.792

6.  Improved electroporation efficiency of intact Lactococcus lactis subsp. lactis cells grown in defined media.

Authors:  D A McIntyre; S K Harlander
Journal:  Appl Environ Microbiol       Date:  1989-10       Impact factor: 4.792

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Authors:  Z Banfalvi; A Nieuwkoop; M Schell; L Besl; G Stacey
Journal:  Mol Gen Genet       Date:  1988-11

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Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205

9.  Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI.

Authors:  H P Spaink; R J Okker; C A Wijffelman; E Pees; B J Lugtenberg
Journal:  Plant Mol Biol       Date:  1987-01       Impact factor: 4.076

10.  Gene transfer into mouse lyoma cells by electroporation in high electric fields.

Authors:  E Neumann; M Schaefer-Ridder; Y Wang; P H Hofschneider
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
  10 in total
  8 in total

1.  Transformation of rhizobia with broad-host-range plasmids by using a freeze-thaw method.

Authors:  Eva Vincze; Steve Bowra
Journal:  Appl Environ Microbiol       Date:  2006-03       Impact factor: 4.792

2.  Rhizobia species: A Boon for "Plant Genetic Engineering".

Authors:  Urmi Patel; Sarika Sinha
Journal:  Indian J Microbiol       Date:  2011-02-26       Impact factor: 2.461

3.  Involvement of multiple loci in quorum quenching of autoinducer I molecules in the nitrogen-fixing symbiont Rhizobium (Sinorhizobium) sp. strain NGR234.

Authors:  D Krysciak; C Schmeisser; S Preuss; J Riethausen; M Quitschau; S Grond; W R Streit
Journal:  Appl Environ Microbiol       Date:  2011-06-03       Impact factor: 4.792

4.  Lipoprotein PssN of Rhizobium leguminosarum bv. trifolii: subcellular localization and possible involvement in exopolysaccharide export.

Authors:  Małgorzata Marczak; Andrzej Mazur; Jarosław E Król; Wiesław I Gruszecki; Anna Skorupska
Journal:  J Bacteriol       Date:  2006-10       Impact factor: 3.490

5.  Genetic dissection of tropodithietic acid biosynthesis by marine roseobacters.

Authors:  Haifeng Geng; Jesper Bartholin Bruhn; Kristian F Nielsen; Lone Gram; Robert Belas
Journal:  Appl Environ Microbiol       Date:  2008-01-11       Impact factor: 4.792

6.  Candidatus Frankia Datiscae Dg1, the Actinobacterial Microsymbiont of Datisca glomerata, Expresses the Canonical nod Genes nodABC in Symbiosis with Its Host Plant.

Authors:  Tomas Persson; Kai Battenberg; Irina V Demina; Theoden Vigil-Stenman; Brian Vanden Heuvel; Petar Pujic; Marc T Facciotti; Elizabeth G Wilbanks; Anna O'Brien; Pascale Fournier; Maria Antonia Cruz Hernandez; Alberto Mendoza Herrera; Claudine Médigue; Philippe Normand; Katharina Pawlowski; Alison M Berry
Journal:  PLoS One       Date:  2015-05-28       Impact factor: 3.240

7.  The Cell Membrane of a Novel Rhizobium phaseoli Strain Is the Crucial Target for Aluminium Toxicity and Tolerance.

Authors:  Clabe Wekesa; John O Muoma; Michael Reichelt; George O Asudi; Alexandra C U Furch; Ralf Oelmüller
Journal:  Cells       Date:  2022-03-03       Impact factor: 6.600

8.  PssJ Is a Terminal Galactosyltransferase Involved in the Assembly of the Exopolysaccharide Subunit in Rhizobium Leguminosarum bv. Trifolii.

Authors:  Małgorzata Marczak; Magdalena Wójcik; Kamil Żebracki; Anna Turska-Szewczuk; Kamila Talarek; Dominika Nowak; Leszek Wawiórka; Marcin Sieńczyk; Agnieszka Łupicka-Słowik; Kamila Bobrek; Marceli Romańczuk; Piotr Koper; Andrzej Mazur
Journal:  Int J Mol Sci       Date:  2020-10-20       Impact factor: 5.923
  8 in total

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