Literature DB >> 2165971

Efficient transformation of Agrobacterium tumefaciens by electroporation.

M Mersereau1, G J Pazour, A Das.   

Abstract

High-voltage electroporation was used to transform Agrobacterium tumefaciens strains A136 and A348, reaching the efficiency of 1-3 x 10(8) transformants/micrograms DNA. Transformation frequency was dependent on the electrical field strength and the pulse length. No significant reduction in transformation efficiency was observed when the transforming DNA contained sites sensitive to endonuclease AtuCI of A. tumefaciens.

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Year:  1990        PMID： 2165971     DOI： 10.1016/0378-1119(90)90452-w

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  68 in total

1.  Plant genome complexity may be a factor limiting in situ the transfer of transgenic plant genes to the phytopathogen Ralstonia solanacearum.

Authors:  F Bertolla; R Pepin; E Passelegue-Robe; E Paget; A Simkin; X Nesme; P Simonet
Journal:  Appl Environ Microbiol       Date:  2000-09       Impact factor: 4.792

2.  High-efficiency transformation of Rhizobium leguminosarum by electroporation.

Authors:  B Garg; R C Dogra; P K Sharma
Journal:  Appl Environ Microbiol       Date:  1999-06       Impact factor: 4.792

3.  Promoter of the rolC gene of Agrobacterium rhizogenes can be strongly regulated in glandular cell of transgenic tobacco.

Authors:  Yuanlei Hu; Bojun Chen; Ting Ni; Ning Li; Zhongping Lin
Journal:  Mol Biotechnol       Date:  2003-06       Impact factor: 2.695

4.  A highly selectable and highly transferable Ti plasmid to study conjugal host range and Ti plasmid dissemination in complex ecosystems.

Authors:  S Teyssier-Cuvelle; P Oger; C Mougel; K Groud; S K Farrand; X Nesme
Journal:  Microb Ecol       Date:  2004-05-28       Impact factor: 4.552

5.  A cooperative virulence plasmid imposes a high fitness cost under conditions that induce pathogenesis.

Authors:  Thomas G Platt; James D Bever; Clay Fuqua
Journal:  Proc Biol Sci       Date:  2011-11-23       Impact factor: 5.349

6.  Development of a simple and efficient system for excising selectable markers in Arabidopsis using a minimal promoter::Cre fusion construct.

Authors:  Hyun-Bi Kim; Jung-Il Cho; Nayeon Ryoo; Shaohong Qu; Guo-Liang Wang; Jong-Seong Jeon
Journal:  Mol Cells       Date:  2011-11-29       Impact factor: 5.034

7.  Properties of Commelina yellow mottle virus's complete DNA sequence, genomic discontinuities and transcript suggest that it is a pararetrovirus.

Authors:  S L Medberry; B E Lockhart; N E Olszewski
Journal:  Nucleic Acids Res       Date:  1990-09-25       Impact factor: 16.971

8.  Motility and chemotaxis in Agrobacterium tumefaciens surface attachment and biofilm formation.

Authors:  Peter M Merritt; Thomas Danhorn; Clay Fuqua
Journal:  J Bacteriol       Date:  2007-08-31       Impact factor: 3.490

9.  A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite.

Authors:  W C Fuqua; S C Winans
Journal:  J Bacteriol       Date:  1994-05       Impact factor: 3.490

10.  Quorum sensing in Rhizobium sp. strain NGR234 regulates conjugal transfer (tra) gene expression and influences growth rate.

Authors:  Xuesong He; William Chang; Deanne L Pierce; Laura Ort Seib; Jennifer Wagner; Clay Fuqua
Journal:  J Bacteriol       Date:  2003-02       Impact factor: 3.490
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